药学学报, 1993, 28(7): 494-498
引用本文:
肖殿模;周文华;张亚海;王小鲁;朱波;张俊宝;杨国栋;陈华粹. (-)棉酚和(+)棉酚对蛋白激酶C及蛋白激酶A活力影响的比较[J]. 药学学报, 1993, 28(7): 494-498.
DM Xiao; WH Zhou; YH Zhang. XL Wang; B Zhu; JB Zhang; GD Yang and HC Chen. COMPARISON BETWEEN THE EFFECIS OF(-)-AND (+)-GOSSYPOL ON PROTEIN KINASE C AND PROTEIN KINASE A[J]. Acta Pharmaceutica Sinica, 1993, 28(7): 494-498.

(-)棉酚和(+)棉酚对蛋白激酶C及蛋白激酶A活力影响的比较
肖殿模;周文华;张亚海;王小鲁;朱波;张俊宝;杨国栋;陈华粹
中国医学科学院基础医学研究所,北京100005;**浙江省宁波微循环与莨菪类药研究所
摘要:
部分纯化的大鼠肝脏蛋白激酶C和蛋白激酶A,在体外与棉酚的旋光异构体分别温育,结果(--)棉酚和(+)棉酚对蛋白激酶C活力均有剂量依赖性的抑制作用;(-)棉酚对Ⅰ型蛋白激酶A活力的抑制作用明显比(+)棉酚强,而(-)棉酚和(+)棉酚在高浓度时对Ⅱ型蛋白激酶A才显示抑制作用。结果提示棉酚在体内可能干扰细胞信息的传递过程。
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COMPARISON BETWEEN THE EFFECIS OF(-)-AND (+)-GOSSYPOL ON PROTEIN KINASE C AND PROTEIN KINASE A
DM Xiao; WH Zhou; YH Zhang. XL Wang; B Zhu; JB Zhang; GD Yang and HC Chen
Abstract:
Protein kinase C(PKC) and protein kinase A(PKA) purified from rat liver were incubated with (-)-and (+)-gossypol. The activity of PKC was significantly inhibited in a concentration-dependent manner by both (-)-gossypol and (+)-gossypol. (-)-gossypol was found to inhibit type Ⅰ PKA, whereas the inhibitory action of (+)-gossypol for type Ⅰ PKA was less potent. Both (-)-gossypol and (+)-gossypol showed inhibition for type Ⅱ PKA only at high concentration. These results suggest that administration of gossypol might impair cellular signal transduction.
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