药学学报, 1992, 27(4): 246-251
引用本文:
齐爱东;吴葆杰;周序斌. 花生四烯酸、二十碳五烯酸及二十二碳六烯酸对兔主动脉条张力的影响[J]. 药学学报, 1992, 27(4): 246-251.
AD Qi;BJ Wu;XB Zhou. EFFECTS OF ARACHIDONIC ACID,EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID ON TENSION OF RABBIT AORTIC STRIPS[J]. Acta Pharmaceutica Sinica, 1992, 27(4): 246-251.

花生四烯酸、二十碳五烯酸及二十二碳六烯酸对兔主动脉条张力的影响
齐爱东;吴葆杰;周序斌
山东医科大学药理教研室,济南250012;*胜利油田卫校山东257035
摘要:
外源性AA引起兔动脉条收缩,呈剂量依赖性;EPA抑制AA收缩血管亦呈浓度依赖性;DHA对AA收缩血管作用无明显影响。破坏血管内皮后AA收缩血管作用大为减弱,EPA抑制AA收缩血管作用也几乎消失。吲哚美辛能阻断AA收缩兔主动脉条的作用。兔主动脉6-keto-PGF、TXB2及其比值随AA浓度升高而增加,低剂量EPA对前列腺素类代谢无明显影响,较大剂量时则降低上述指标。
关键词:   
EFFECTS OF ARACHIDONIC ACID,EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID ON TENSION OF RABBIT AORTIC STRIPS
AD Qi;BJ Wu;XB Zhou
Abstract:
The purpose of the investigation was to determine the effects ofeicosapentaenoic acid (EPA), docosahexaenoic acid (DHA)and arachidonic acid (AA)on the isolated rabbit aortic strips. The results showed that (1) the rabbit aortic strips withintact endothelium contracted in a dose-dependent manner to exogenous AA betweenthe concentrations of 2.5 ×10-6 mol/L and 4 ×10-5mol/L. EPA did not affect the tensionof the relaxing strips, but inhibited the contractile response to AA (2×10-5mol/L), IC50=9.94×10-9 mol/L. DHA showed no significant effect on the contractile response to AA.(2) In the endothelium- damaged rabbit aorta, the contraction of strips to AA was drasti-cally diminished. The inhibitory effect of EPA on the contraction to AA was almost van-ished. (3)The contraction of rabbit aortic strips to AA was shown to be abolished byindomethacin ( 1.5 ×10-5mol/L), a cyclooxygenase inhibitor .(4) Radioimmunoassayshowed that exogenous AA increased the tissue levels of 6- keto- PGF, TXB2 and theirratio. Lower dose of EPA( 1.5 ×10-6 or 1.5 ×10-5 mol/L)did not affect the tissue levels of6- keto - PGF and TXB2. However, EPA at higher concentrations decreased the meta-bolites of AA significantly. These findings suggest that exogenous AA induces the contraction of rabbit aortic strips in vitro,which is related to the endothelial cells and mediated by the metabolites(s) ofcyclooxygenase,likely endothelium- derived contracting factor(EDCF). EPA could inhibitthe contraction of rabbit aortic strips to AA,which depends upon endothelium and is possi-bly evoked through augmenting synthesis and release of endothelium- derived relaxing fac-tor(EDRF). DHA exhibited no influence on the AA induced vasocontraction.
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