药学学报, 2012, 47(10): 1341-1346
引用本文:
刁振宇, 卢悟广, 曹 鹏, 胡云龙, 周 星, 薛平平, 沈 莉, 孙海翔. EGFR纳米抗体抑制雌激素依赖的人子宫内膜癌 Ishikawa细胞的增殖与迁移[J]. 药学学报, 2012, 47(10): 1341-1346.
DIAO ZY,LU WG,CAO Peng,HU Yun-long,ZHOU Xing,XUE Ping-ping,SHEN Li,SUN Hai-xiang. AntiEGFRnano inhibites proliferation and migration of estrogen-dependent Ishikawa cells of human endometrial cancer cell line[J]. Acta Pharmaceutica Sinica, 2012, 47(10): 1341-1346.

EGFR纳米抗体抑制雌激素依赖的人子宫内膜癌 Ishikawa细胞的增殖与迁移
刁振宇1, 卢悟广2, 曹  鹏2, 胡云龙3, 周  星3, 薛平平1, 沈  莉1, 孙海翔1*
(1. 南京大学医学院附属鼓楼医院生殖医学中心, 江苏 南京 210008; 2. 江苏省中医药研究院细胞与分子生物学实验室, 江苏 南京 210028; 3. 南京川博生物技术有限公司, 江苏 南京 210061)
摘要:

纳米抗体是骆驼来源的一种轻链缺失的抗体, 具有与单克隆抗体相似的抗原结合特异性和亲和力, 此外还具有分子量小、稳定性好、易于制备等优点, 因而具有巨大的生物医药应用价值。本研究利用基因工程技术在大肠杆菌中成功制备出了高纯度的EGFR纳米抗体 (antiEGFRnano)CCK-8法检测细胞增殖及迁移实验 (细胞划痕实验和Transwell法检测) 证明, 重组的EGFR纳米抗体具有显著的抑制子宫内膜癌细胞增殖及迁移的活性, 这为靶向EGFR治疗子宫内膜癌提供了一种全新的思路和方法。

关键词:   
AntiEGFRnano inhibites proliferation and migration of estrogen-dependent Ishikawa cells of human endometrial cancer cell line
Abstract:

Nanobody is a kind of antibody from camel, which misses light chain.  Nanobody has the same antigen binding specificity and affinity as mAb.  Moreover, because of its small molecular weight, high stability and easy preparation, nanobody has great value of biomedical applications.  In this study, we successfully prepared highly pure antiEGFR nanobody in E.coli using genetic engineering techniques.  Cell proliferation assay (CCK-8 assay) and migration experiments (cell scratch test and Transwell assay) indicated that the recombinant antiEGFRnano can significantly inhibit the proliferation and migration of endometrial cancer cells.  These results provide a new way of thinking and methods for EGFR-targeted therapy of endometrial cancer.

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