药学学报, 2012, 47(10): 1375-1383
引用本文:
史旭芹, 尚尔鑫, 唐于平, 朱华旭, 郭建明, 黄美艳, 李伟霞, 段金廒. 基于响应曲面分析法对当归-黄芪配伍养血补血功效相互作用研究[J]. 药学学报, 2012, 47(10): 1375-1383.
. Interaction of nourishing and tonifying blood effects of the combination of Angelicae sinensis Radix and Astragali Radix studied by response surface method[J]. Acta Pharmaceutica Sinica, 2012, 47(10): 1375-1383.

基于响应曲面分析法对当归-黄芪配伍养血补血功效相互作用研究
史旭芹, 尚尔鑫, 唐于平*, 朱华旭, 郭建明, 黄美艳, 李伟霞, 段金廒
(南京中医药大学, 江苏省方剂高技术研究重点实验室, 江苏 南京 210046)
摘要:

采用响应曲面设计与分析法, 定量研究当归黄芪配伍养血补血功效的相互作用。采用乙酰苯肼和环磷酰胺复合法复制小鼠血虚模型, 灌胃给予当归黄芪不同配比 (011525231132525110) 的提取物, 以外周血常规和脏器指数为检测指标, 通过多指标综合指数法对各指标数值进行整合, 应用Matlab软件构建当归黄芪配伍整合效应的三维响应曲面图, 并进行相互作用分析。结果显示, 当归-黄芪配伍接近一半区域呈现出协同效应, 在所测试的比例范围 (1551) 均有协同效应表现, 总体表现为高剂 量组的协同作用强于低剂量组, 尤其是当归剂量在1040 g、黄芪剂量在90180 g区间以及当归剂量在50100 g、黄芪剂量在20100 g区间表现出很强的协同效应 (相互作用值: 1)。在中国药典规定剂量范围 (当归: 612 g, 黄芪: 930 g) 也均呈现出一定的协同作用, 其中当归补血汤 (当归: 6 g, 黄芪: 30 g) 亦在此范围内。本实验结果为中医临床合理配伍应用当归与黄芪提供了科学依据; 并首次尝试运用响应曲面分析法对中药配伍效应变化进行定量评价, 为方药配伍相互作用现代基础研究提供了思路与方法。

关键词:   
Interaction of nourishing and tonifying blood effects of the combination of Angelicae sinensis Radix and Astragali Radix studied by response surface method
Abstract:

The combination of Angelicae sinensis Radix (Danggui, DG) and Astragali Radix (Huangqi, HQ) is a popular herb pair commonly used in clinic for the treatment of blood deficiency syndrome in China.  The aim of this paper is to study the interaction of DG and HQ nourishing and tonifying blood effects by response surface method.  The blood deficiency mice were induced by injecting N-acetylphenylhydrazine (sc) and cyclophosphamide (ip).  The blood deficiency mice were administrated intragastrically with DG-HQ extracts (01, 15, 25, 23, 11, 32, 52, 51, 10).  The changes of the peripheral blood indexes and organ indexes were observed.  The indexes were integrated by comprehensive index method; the interactions of DG and HQ were analyzed by the response surface diagram established with Matlab software.  The results showed that DG and HQ at most of their combination ratios had synergic effect.  Within the range of 15 51, all of the extracts of DG-HQ showed synergic effect, and among which, high-doses had better effects than low-doses.  The highest value (1) of the synergic effect was showed when DG was 10 40 g at the same time of HQ as 90 180 g, and DG was 50 100 g at the same time of HQ as 20 100 g.  DG-HQ at all combination dosages within Chinese Pharmacopeia (DG: 6 12 g, HQ: 9 30 g) had certain synergic effect, and Danggui Buxue Decoction (DG: 6 g, HQ: 30 g) also was at this range.  The results provided scientific basis to the clinical application of DG and HQ.  And the response surface method was firstly applied to quantitatively evaluate the bio-activity change of herb combination, which provided a novel way for modern basic research on the interaction of herbs.

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