药学学报, 2012, 47(10): 1399-1402
引用本文:
罗 璐, 赵淑娟, 王峥涛, 胡之璧. 人雌激素受体ERα/ERβ在大肠杆菌中的表达[J]. 药学学报, 2012, 47(10): 1399-1402.
LUO Lu, ZHAO Shu-juan, WANG Zheng-tao, HU Zhi-bi. Expression of human estrogen receptor α and β in Escherichia coli[J]. Acta Pharmaceutica Sinica, 2012, 47(10): 1399-1402.

人雌激素受体ERα/ERβ在大肠杆菌中的表达
罗  璐, 赵淑娟*, 王峥涛, 胡之璧
(上海中医药大学中药研究所, 国家中医药管理局中药新资源和品质评价重点研究室, 上海复方中药重点实验室, 上海 201203)
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Expression of human estrogen receptor α and β in Escherichia coli
Abstract:

Estrogen participates in many life activities through combination with estrogen receptor α (ERα) or estrogen receptor β (ERβ) in the body.  In order to establish an in vitro estrogen-like compound screening model, the coding region of human ERα and ERβ was separately constructed into pET32-ERα and pET43-ERβ prokaryotic expression vector and water-soluble recombinant ERα and ERβ proteins were expressed in Escherichia coli strain BL21.  Western blotting revealed that both recombinant proteins have estrogen receptor binding sites.  The proteins were purified using S•Tag affinity Purification Kit and digested with enterokinase to get the ERα and ERβ proteins.  About 0.90 mg of ERα and 0.65 mg of ERβ were obtained at the concentration of 0.181 and 0.131 mg·mL−1, respectively.

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