药学学报, 2013, 48(6): 834-841
引用本文:
卢多. C2H2型锌指对生物大分子的识别[J]. 药学学报, 2013, 48(6): 834-841.
LU Duo. C2H2 zinc-finger recognition of biomolecules[J]. Acta Pharmaceutica Sinica, 2013, 48(6): 834-841.

C2H2型锌指对生物大分子的识别
卢 多*
(中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050)
摘要:

C2H2锌指结构域在3% 人类基因组编码蛋白中出现, 可以特异性识别DNARNA和蛋白质。经过近30年的研究, 锌指识别生物大分子的作用机制得到不同程度的解释。在识别DNA方面, 锌指结合在DNA双螺旋的大沟中, 并在氨基酸与碱基之间一一对应, 根据DNA序列的不同实现特异性识别。在识别RNA方面, 针对RNA结构上的多样性, 锌指综合识别特异碱基与特异的折叠骨架。在识别蛋白质方面, 作用机制的研究尚处初始阶段, 现有的研究结果显示为多元化的识别方式。分子作用机制的理论研究又催生了工程锌指蛋白, 并且大量地进入实际应用。鉴于锌指在自然界的普遍存在、在功能上的多样性及在转化应用上的潜力, 有必要对其进行系统的研究。

关键词:   
C2H2 zinc-finger recognition of biomolecules
Abstract:

C2H2 zinc-finger motif presents in 3% of proteins that are encoded in the human genome, and has the abilities to recognize DNA, RNA and protein.  With nearly 3 decades of efforts, the mechanisms of zinc-finger mediated biomolecule recognitions have been studied to various extents.  Zinc-finger binds into the major groove of DNA double helix, establishes an one-to-one recognition format between DNA bases and certain amino acids in a zinc-finger, and achieves specificity based on DNA sequences.  While RNA molecules show a large variety in their structures, zinc-finger recognizes RNA through the collected information of specially displayed bases and special backbone folding.  Initial studies have been performed on zinc-finger mediated protein-protein interactions.  Existing data indicate multiple recognition modes.  The studies on molecular mechanism have supported the development of engineered zinc-fingers, which have been introduced into applications.  For its wide existence, large functional diversity and potential in translational applications, zinc-finger deserves a systematic study in every aspect.

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