药学学报, 2013, 48(6): 906-910
引用本文:
吕允凤. 应用微透析技术和高效液相色谱法测定川芎嗪对大鼠脑内多巴胺释放量的影响[J]. 药学学报, 2013, 48(6): 906-910.
Lü Yun-feng. Effects of ligustrazine on extracellular dopamine levels in rat brain dialysate[J]. Acta Pharmaceutica Sinica, 2013, 48(6): 906-910.

应用微透析技术和高效液相色谱法测定川芎嗪对大鼠脑内多巴胺释放量的影响
吕允凤*
(国家食品药品监督管理局医疗器械技术审评中心, 北京 100044)
摘要:

 利用脑内微透析技术与现代分析方法, 考察了不同剂量川芎嗪对大鼠脑内多巴胺释放的影响。采用脑内微透析技术进行在体动态取样, 建立了高效液相电化学检测方法测定脑透析液中的多巴胺含量。结果显示川芎嗪皮下给药能够剂量相关性地增加大鼠脑内不同脑区中多巴胺的释放。该方法能够在不影响动物正常生理活动的情况下准确反映药物对大鼠脑内多巴胺释放量的影响, 与传统神经递质研究方法相比, 具有明显的优势。

关键词:   
Effects of ligustrazine on extracellular dopamine levels in rat brain dialysate
Abstract:

Using brain microdialysis and LC-ECD, the content of dopamine in rat brain was detected to investigate the effects of ligustrazine.  A liquid chromatography-electrochemical detector method has been established and validated for the determination of dopamine in rat brain dialysate.  The results indicate that ligustrazine administration by subcutaneous injection significantly increased dopamine release in rat medial prefrontal cortex, nucleus accumbens and hippocampus in a dose-related manner.  The drug’s effects on dopa release in rat brain could be directly detected by microdialysis combined with HPLC-ECD and this method has the preponderance over traditional neurology methods.

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