药学学报, 2013, 48(6): 960-964
引用本文:
周 建, 葛宝丰, 陈克明, 马小妮, 成 魁, 郭晓宇, 吕 享. 金雀异黄酮对体外培养大鼠股骨组织骨代谢活性的影响[J]. 药学学报, 2013, 48(6): 960-964.
ZHOU Jian, GE Bao-feng,CHEN Ke-ming, MA Xiao-ni, CHENG Kui, GUO Xiao-yu,Lü Xiang. Effect of genistein on rat femoral bone metabolic activity in vitro[J]. Acta Pharmaceutica Sinica, 2013, 48(6): 960-964.

金雀异黄酮对体外培养大鼠股骨组织骨代谢活性的影响
周 建, 葛宝丰, 陈克明*, 马小妮, 成 魁, 郭晓宇, 吕 享
(兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050)
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Effect of genistein on rat femoral bone metabolic activity in vitro
Abstract:

This study is to investigate effects of genistein on rat femoral bone metabolic in vitro.  Rat femoral tissues was isolated and randomly divided into two groups including control group and genistein (1×10−5 mol·L−1) group.  Determinations of alkaline phosphatase (ALP) activity, calcium content and osteoprotegerin (OPG), type I-collagen (Collagen-I), RANKL, Runx-2 and bone morphogenetic protein (BMP-2) mRNA expression were done by real-time PCR.  The results showed that 1×10−5 mol·L−1 genistein could increase the activity of ALP and contents of Ca, regulate bone metabolism activity of OPG, RANKL, BMP-2, Collagen-I and Runx-2 mRNA expression level.  Genistein can significantly modulate bone metabolism related gene expression level of rat femoral tissue in vitro, and can increase calcium content and the activity of ALP.

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