药学学报, 2013, 48(8): 1301-1306
引用本文:
李伟霞 黄美艳 唐于平 郭建明 尚尔鑫 王林艳 钱大玮 段金廒. 基于代谢组学研究佛手散对血虚小鼠的养血补血作用机制[J]. 药学学报, 2013, 48(8): 1301-1306.
LI Wei-Xia, Huang Mei-Yan, Tang Xu-ping, Guo Jian-Ming, Shang Er-Xin, Wang Lin-Yan, Qian Da-Wei, Duan Jin-Ao. Metabolomic study of the action mechanism of nourishing blood effect of Fo-Shou-San on blood deficiency mice[J]. Acta Pharmaceutica Sinica, 2013, 48(8): 1301-1306.

基于代谢组学研究佛手散对血虚小鼠的养血补血作用机制
李伟霞1, 黄美艳1, 2, 唐于平1*, 郭建明1, 尚尔鑫1, 王林艳1, 钱大玮1, 段金廒1
(1. 南京中医药大学, 江苏省方剂高技术研究重点实验室, 江苏 南京 210023; 2. 普洱市人民医院临床药学室, 云南 普洱 665000)
摘要:

运用代谢组学方法研究佛手散对血虚小鼠代谢的作用机制。采用乙酰苯肼和环磷酰胺联合使用复制血虚小鼠模型, 通过UPLC-QTOF/MS分析血虚小鼠血浆样品的代谢指纹图谱, 质谱数据采用MarkerLynx软件处理, 然后采用偏最小二乘判别法分析正常组、血虚模型组和佛手散给药组之间的代谢物差异, 并通过变量重要性投影选取潜在的生物标志物, 结合质谱信息和数据库检索对潜在的生物标志物进行鉴定, 将鉴定到的生物标志物输入MetPA数据库中构建代谢通路。结果表明, 3组小鼠的血浆代谢物谱得到了很好的区分, 发现并鉴定了22个潜在生物标志物, 这些生物标志物主要与硫胺代谢、花生四烯酸代谢、鞘脂类代谢、乙醛酸和二羧酸根阴离子代谢、组氨酸代谢、烟酸和烟酰胺代谢、半胱氨酸和蛋氨酸代谢、色氨酸代谢、糖代谢、酪氨酸代谢和柠檬酸循环等11条代谢通路相关。这些代谢通路在血虚小鼠体内发生紊乱, 而给予佛手散后可使之逆转并向正常状态转归。该研究对血虚小鼠模型代谢组及佛手散的养血补血作用机制进行了阐释, 说明采用代谢组学方法能整体反映生物体的生理及代谢状态, 可用于中药及方剂药效评价与相关机制研究。

关键词:   
Metabolomic study of the action mechanism of nourishing blood effect of Fo-Shou-San on blood deficiency mice
Abstract:

The metabolic effect of Fo-Shou-San on blood deficiency mice was studied by using metabolomic method.  UPLC-QTOF/MS was used to analyze the plasma metabolome in blood deficiency mice.  MS data were processed by MarkerLynx software.  With multivariate statistical analysis of plasma metabolite profiles, a clear separation among control, blood deficiency model, and Fo-Shou-San groups was achieved.  Potential biomarkers were selected according to the parameters of variable importance in the projection (VIP) and identified according to MS information and database retrieval.  The metabolic network of blood deficiency was predicted via MetPA database.  Twenty-two potential biomarkers were identified and used to explain the thiamine metabolism, arachidonic acid metabolism, sphingolipid metabolism, glyoxylate and dicarboxylate metabolism, histidine metabolism, nicotinate and nicotinamide metabolism, cysteine and methionine metabolism, tryptophan metabolism, starch and sucrose metabolism, tyrosine metabolism and citrate cycle (TCA cycle).  Those metabolic pathways were disturbed in blood deficiency mice, but which could be regulated nearly to normal state after Fo-Shou-San administration.  In this study, the metabolomics of blood deficiency mice and the action mechanism of nourishing blood effect of Fo-Shou-San were evaluated.  The physiological and metabolic state of the organism could be represented comprehensively by using metabolomics.  And metabolomics can be used to evaluate the pharmacodynamics and related mechanisms of Chinese medicine and formulae.

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