药学学报, 2014, 49(8): 1130-1135
引用本文:
谢欣梅, 庞晓斌, 赵艳, 王保全, 陈若芸, 杜冠华. 消栓通络有效成分组对氧糖剥夺损伤原代培养神经元的保护作用[J]. 药学学报, 2014, 49(8): 1130-1135.
XIE Xin-mei, PANG Xiao-bin, ZHAO Yan, WANG Bao-quan, CHEN Ruo-yun, DU Guan-hua. Neuroprotective effects of the effective components group of Xiaoshuantongluo against oxygen-glucose deprivation in primary cultured rat cortical neurons[J]. Acta Pharmaceutica Sinica, 2014, 49(8): 1130-1135.

消栓通络有效成分组对氧糖剥夺损伤原代培养神经元的保护作用
谢欣梅1, 庞晓斌1, 赵艳2, 王保全3, 陈若芸4, 杜冠华4
1. 河南大学药物研究所, 河南 开封 475004;
2. 青岛市市立医院, 山东 青岛 266011;
3. 河南大学淮河临床学院, 河南 开封 475001;
4. 中国医学科学院、北京协和医学院药物研究所国家药物筛选中心, 北京 100050
摘要:
研究消栓通络有效成分组(XECG) 对原代培养皮质神经元氧糖剥夺(OGD) 损伤的保护作用,并探讨其可能的作用机制。采用3日龄SD大鼠皮质制备原代培养神经元;将细胞随机分为正常对照组、OGD模型组和XECG组(1、3及10 mg·L-1);四甲基偶氮唑盐(MTT) 法测定细胞存活率;酶标法试剂盒测定乳酸脱氢酶(LDH) 漏出量;Hoechst染色检测细胞凋亡;RT-PCR检测JAK2、STAT3 mRNA表达变化;Western blotting检测Bcl-2、Bax、p-JAK2、p-STAT3蛋白表达变化。结果显示,XECG能明显改善OGD对神经元的损伤,提高细胞存活率,减少LDH的释放量,抑制缺氧缺糖引起的细胞凋亡;能提高缺糖缺氧神经元Bcl-2/Bax比值,增加JAK2、STAT3基因和蛋白的表达。以上结果表明,XECG对OGD损伤的原代培养神经元具有明显保护作用,其机制可能与激活JAK2/STAT3通路、影响凋亡相关基因Bcl-2及Bax的表达有关。
关键词:    消栓通络有效成分组 (XECG)       原代培养神经元      糖氧剥夺      JAK2/STAT3信号通路     
Neuroprotective effects of the effective components group of Xiaoshuantongluo against oxygen-glucose deprivation in primary cultured rat cortical neurons
XIE Xin-mei1, PANG Xiao-bin1, ZHAO Yan2, WANG Bao-quan3, CHEN Ruo-yun4, DU Guan-hua4
1. Pharmaceutical Institute, Henan University, Kaifeng 475004, China;
2. Qingdao Municipal Hospital, Qingdao 266011, China;
3. The Huaihe Hospital of Henan University, Kaifeng 475001, China;
4. National Centre for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Abstract:
This study is to investigate the effect of the effective components group of Xiaoshuantongluo (XECG) on neuronal injury induced by oxygen-glucose deprivation (OGD) in primary cortical cultures isolated from SD rat cortex at day 3 and the possible mechanism. Cells were divided into control group, OGD model group and XECG group (1, 3 and 10 mg·L-1). The cell viability was assessed with MTT assay and the LDH release rate was measured by enzyme label kit. The cell apoptosis was analyzed using Hoechst staining. RT-PCR was applied to detect the mRNA levels of JAK2 and STAT3. Western blotting was used to detect the expressions of Bcl-2, Bax, p-JAK2 and p-STAT3 proteins. Results showed that XECG resulted in an obvious resistance to oxygen-glucose deprivation-induced cell apoptosis and decrement of cell viability, decrease the cell LDH release rate. XECG could adjust the expression of Bcl-2 and Bax proteins and increase Bcl-2/Bax ratio, up-regulate the expression of p-JAK2 and p-STAT3. In conclusion, XECG could protect against the neuronal injury cells exposed to OGD, which may be relevant to the promotion of JAK2/STAT3 signaling pathway, and impact the expression of Bax and Bcl-2.
Key words:    XECG    primary neuronal culture    oxygen-glucose deprivation    JAK2/STAT3 signaling pathway   
收稿日期: 2014-03-27
基金项目: 国家自然科学基金资助项目(81273652).
通讯作者: 庞晓斌 Tel/Fax:86-371-23880680,E-mail:hndxpxb@163.com
Email: hndxpxb@163.com
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