药学学报, 2014, 49(8): 1150-1154
引用本文:
张晶, 孙桂波, 雷崎方, 李广志, 王钧篪, 斯建勇. 生附子的化学成分研究[J]. 药学学报, 2014, 49(8): 1150-1154.
ZHANG Jing, SUN Gui-bo, LEI Qi-fang, LI Guang-zhi, WANG Jun-chi, SI Jian-yong. Chemical constituents of lateral roots of Aconitum carmichaelii Debx[J]. Acta Pharmaceutica Sinica, 2014, 49(8): 1150-1154.

生附子的化学成分研究
张晶, 孙桂波, 雷崎方, 李广志, 王钧篪, 斯建勇
中国医学科学院、北京协和医学院药用植物研究所, 北京 100193
摘要:
为研究中药附子强心活性的物质基础,对其化学成分进行了研究。采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、MCI、ODS等柱色谱及制备液相色谱等手段从附子90%乙醇提取物中分离得到10个化合物,根据化合物的理化性质和波谱数据鉴定了其结构,分别为5-羟基-2-苯甲酰胺-苯甲酸甲酯 (1)、和厚朴酚 (2)、松脂醇 (3)、水杨酸 (4)、对羟基桂皮酸 (5)、松果灵 (6)、多根乌头碱 (7)、新乌头碱 (8)、次乌头碱 (9) 和苯甲酰次乌头碱 (10)。化合物1为一个新的化合物,命名为附子酰胺(aconitamide),并通过核磁、质谱、紫外、红外及单晶衍射数据确定了其结构。化合物2~5为首次从附子中分离得到。
关键词:    附子      化学成分      附子酰胺     
Chemical constituents of lateral roots of Aconitum carmichaelii Debx
ZHANG Jing, SUN Gui-bo, LEI Qi-fang, LI Guang-zhi, WANG Jun-chi, SI Jian-yong
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
Abstract:
In order to find the cardiotonic constituents of lateral roots of Aconitum carmichaelii Debx., the investigation was carried out. Silica gel column chromatography, Sephadex LH-20, medium-pressure MCI and reverse phase ODS column chromatography were used to separate the 90% EtOH extract of the lateral roots of Aconitum carmichaelii Debx. The structures of the isolated compounds have been identified by chemical properties and spectroscopic analyses. Ten compounds were isolated and their structures were elucidated as benzoic acid-5-hydroxy-2-benzoyl-amino methyl ester (1), honokiol (2), pinoresinol (3), salicylic acid (4), p-hydroxy-cinnamic acid (5), songorine (6), karakoline (7), mesaconitine (8), hypaconitine (9) and 14-benzoylhypaconitine (10), separetely. Compound 1 is a new compound and its structure has been established by NMR, HR-ESI-MS, UV, IR and X-Ray. Compound 2-5 are isolated from the lateral roots of Aconitum carmichaelii Debx. for the first time.
Key words:    lateral roots of Aconitum carmichaelii Debx.    constituent    aconitamide   
收稿日期: 2014-02-24
基金项目: 国家重点基础研究发展计划(973计划) 资助项目(2009CB522805);国家重大新药创制科技重大专项(2011ZX09307-002-01,2012ZX09301-001-001).
通讯作者: 斯建勇 Tel/Fax:86-10-57833299,E-mail:jysi@implad.ac.cn
Email: jysi@implad.ac.cn
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