药学学报, 2014, 49(8): 1162-1168
引用本文:
张蔚, 江曙, 钱大玮, 尚尔鑫, 管汉亮, 任浩, 朱振华, 段金廒. 芒柄花苷与人体肠道细菌的相互作用研究[J]. 药学学报, 2014, 49(8): 1162-1168.
ZHANG Wei, JIANG Shu, QIAN Da-wei, SHANG Er-xin, GUAN Han-liang, REN Hao, ZHU Zhen-hua, DUAN Jin-ao. The interaction between ononin and human intestinal bacteria[J]. Acta Pharmaceutica Sinica, 2014, 49(8): 1162-1168.

芒柄花苷与人体肠道细菌的相互作用研究
张蔚, 江曙, 钱大玮, 尚尔鑫, 管汉亮, 任浩, 朱振华, 段金廒
南京中医药大学, 江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心, 江苏 南京 210023
摘要:
研究人体肠道细菌对芒柄花苷的代谢和芒柄花苷对人体肠道细菌生长的影响。芒柄花苷与人体多种肠道细菌共孵育,采用UPLC-Q-TOF/MS技术与MetabolynxTM软件分析芒柄花苷经人体肠道细菌作用后的代谢产物;采用选择性培养基分别培养4类细菌(肠球菌、肠杆菌、双歧杆菌和乳酸杆菌),并采用光比浊法测定加入不同浓度芒柄花苷溶液后这4类细菌的生长变化,从而推测芒柄花苷对人体肠道菌群生长的影响。从药物-细菌孵育液中鉴定出芒柄花苷的去甲基化产物、羟基化产物、氢化产物及去糖基化产物等7种代谢产物;芒柄花苷加入后会抑制病原菌肠球菌和肠杆菌的生长,促进有益菌双歧杆菌和乳酸杆菌的生长。结果提示,肠道细菌对芒柄花苷有代谢作用且由不同细菌完成,同时芒柄花苷对不同种类肠道细菌的生长有影响。
关键词:    芒柄花苷      肠道细菌      代谢产物     
The interaction between ononin and human intestinal bacteria
ZHANG Wei, JIANG Shu, QIAN Da-wei, SHANG Er-xin, GUAN Han-liang, REN Hao, ZHU Zhen-hua, DUAN Jin-ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
Abstract:
The study aims to screen the ability of the bacteria to metabolize ononin and assess the effect of ononin on the intestinal bacteria. Fresh human fecal sample was obtained from a healthy volunteer, diluted serially in sterile water and sixty-nine different bacterial colonies were picked out ultimately. UPLC-Q-TOF/MS with automated data analysis software (MetaboLynxTM) was applied to fast analysis of ononin metabolites. Furthermore, an Emax precision microplate reader was employed to determine the growth situation of Enterococcous sp., Enterobacter sp., Lactobacilli sp., and Bifidobacteria sp. Results indicated that hydrogenation, demethylation, hydroxylation and deglycosylation were the major metabolic pathways of ononin by human intestinal bacteria in vitro. Ononin can inhibit the growth of pathogen such as Enterococcus sp., Enterobacter sp. and can promote the growth of probiotics such as Bifidobacteria sp. and Lactobacilli sp. This study suggested that intestinal bacteria have the metabolic effects of ononin and the biotransformation was completed by different bacteria. And ononin can affect the balance of intestinal flora and the degree of influence varies depending on the bacterial species and the concentration of ononin.
Key words:    ononin    intestinal bacteria    metabolite   
收稿日期: 2014-01-23
基金项目: 国家重点基础研究发展计划(973计划) 资助项目(2011CB505300,2011CB505303);国家科技支撑计划(2011BAI04B03).
通讯作者: 钱大玮 Tel/Fax:86-25-85811916,E-mail:qiandwnj@126.com;段金廒 Tel/Fax:86-25-85811116,E-mail:dja@njutcm.edu.cn
Email: qiandwnj@126.com;dja@njutcm.edu.cn
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