药学学报, 2014, 49(9): 1304-1309
引用本文:
李淑娇, 李伟霞, 唐于平, 沈娟, 尚尔鑫, 郭建明, 段金廒. 基于主成分分析和多指标综合指数法的当归-红花不同配比活血化瘀作用比较[J]. 药学学报, 2014, 49(9): 1304-1309.
LI Shu-jiao, LI Wei-xia, TANG Yu-ping, SHEN Juan, SHANG Er-xin, GUO Jian-ming, DUAN Jin-ao. Comparative analysis of the promoting blood effects of the combination of different proportions of Danggui and Honghua by the principal component analysis and multi-attribute comprehensive index methods[J]. Acta Pharmaceutica Sinica, 2014, 49(9): 1304-1309.

基于主成分分析和多指标综合指数法的当归-红花不同配比活血化瘀作用比较
李淑娇, 李伟霞, 唐于平, 沈娟, 尚尔鑫, 郭建明, 段金廒
南京中医药大学江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物 国家地方联合工程研究中心, 江苏省方剂高技术研究重点实验室, 江苏 南京 210023
摘要:
比较评价当归-红花 (GH) 不同配比对急性血瘀大鼠血液流变学和凝血功能的影响,优选当归-红花配伍活血作用的最佳配比。采用皮下注射盐酸肾上腺素和冰水游泳共同复制急性血瘀大鼠模型,通过测定全血黏度 (WBV)、血浆黏度 (PV)、全血高切相对指数 (HSWBRI)、全血低切相对指数 (LSWBRI)、红细胞聚集指数 (EAI),观察当归-红花配伍对血瘀大鼠血液流变学的影响; 通过测定凝血酶原时间 (PT)、凝血酶时间 (TT)、活化部分凝血活酶时间 (APTT)、血浆纤维蛋白原 (FIB) 含量及血小板最大聚集率 (ADP),观察当归-红花配伍对血瘀大鼠凝血功能及血小板聚集的影响。然后采用主成分分析法和多指标综合指数法综合评价当归-红花配伍的总活血化瘀效应。结果显示,与正常组比较,模型组各血液流变学指标和凝血功能指标均有显著性差异; 与模型组比较,当归-红花不同配比 (1:0、4:1、2:1、3:2、1:1、2:3、1:2、1:4和0:1) 组均能较好改善急性血瘀大鼠的血液流变学各指标,对凝血功能多个指标也有较好的调节作用。主成分分析法和多指标综合指数法综合评价得出当归-红花配比1:1和3:2的活血化瘀作用最好,其中1:1的活血化瘀作用更优于3:2。以上结果说明,当归-红花配伍能明显改善血瘀大鼠的血液流变学及凝血功能异常,且当归-红花活血化瘀作用的最佳配比与临床中医方剂中使用当归-红花1:1频次最高的结论相一致,为临床更有效应用当归-红花配伍提供了科学依据。
关键词:    当归      红花      配伍      活血化瘀      主成分分析      多指标综合指数法     
Comparative analysis of the promoting blood effects of the combination of different proportions of Danggui and Honghua by the principal component analysis and multi-attribute comprehensive index methods
LI Shu-jiao, LI Wei-xia, TANG Yu-ping, SHEN Juan, SHANG Er-xin, GUO Jian-ming, DUAN Jin-ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China
Abstract:
The combination of Danggui and Honghua (GH) is a popular herb pair commonly used in clinic for the treatment of blood stasis syndrome in China. To evaluate the activating blood circulation and dissipating blood stasis effects of the combination of different proportions of Danggui and Honghua on acute blood stasis rats, and optimize the proportion of GH to have the best activating blood circulation and dissipating blood stasis effect. Acute blood stasis rat model was induced by subcutaneous injection of adrenaline and ice water bath. The blood stasis rats were administrated intragastrically with GH (1:0, 4:1, 2:1, 3:2, 1:1, 2:3, 1:2, 1:4 and 0:1) extracts. The whole blood viscosity (WBV), plasma viscosity (PV), and high shear whole blood relative index (HSWBRI), low shear whole blood relative index (LSWBRI), and erythrocyte aggregation index (EAI) were tested to observe the effects of GH on hemorheology of blood stasis rats. And the maximum aggregation induced by adenosine diphosphate (ADP) was tested to observe the effect of GH on platelet aggregation index of blood stasis rats. In addition, the prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (APTT) and plasma fibrinogen (FIB) were tested to observe the effects of GH on blood coagulation function of blood stasis rats. Then principal component analysis and multi-attribute comprehensive index methods were both used to comprehensively evaluate the total activating blood circulation and dissipating blood stasis effects of GH. The results showed that the hemorheological indexes and coagulation parameters of model group both had significant differences with normal group. Compared with model group, GH (1:0, 4:1, 2:1, 3:2, 1:1, 2:3, 1:2, 1:4 and 0:1) could improve all the blood hemorheology indexes and regulate part indexes of blood coagulation function and platelet aggregation in acute blood stasis rats. Based on principal component analysis and multi-attribute comprehensive index methods, GH 1:1 and GH 3:2 both had the best effect of blood circulation and dissipating blood stasis, and the effect of GH 1:1 was slightly better than GH 3:2. These results suggest that GH could obviously ameliorate the abnormality of hemorheology and blood coagulation function in acute blood stasis rats. The optimized proportion of GH was consistent with regulations of medicine usage that GH 1:1 had the highest frequency used in traditional Chinese formulae. It could provide scientific basis for more effective application of the compatibility between Danggui and Honghua in modern clinic medicine.
Key words:    Danggui    Honghua    compatibility    blood circulation and dissipating blood stasis    principal component analysis    multi-attribute comprehensive index method   
收稿日期: 2014-01-10
基金项目: 国家自然科学基金资助项目(81274058);江苏高校优势学科中药学学科建设工程资助项目(PAPD)
通讯作者: 唐于平
Email: yupingtang@njutcm.edu.cn
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