药学学报, 2014, 49(10): 1395-1399
引用本文:
李莫涵, 张晓琳, 叶菲. 小鼠脂肪性肝病超声影像学检测方法的建立[J]. 药学学报, 2014, 49(10): 1395-1399.
LI Mo-han, ZHANG Xiao-lin, YE Fei. Establishment of an ultrasonographic method for evaluating the hepatic lipid accumulation in mice[J]. Acta Pharmaceutica Sinica, 2014, 49(10): 1395-1399.

小鼠脂肪性肝病超声影像学检测方法的建立
李莫涵, 张晓琳, 叶菲
中国医学科学院、北京协和医学院药物研究所, 新药作用机制研究与药效评价北京市重点实验室, 北京 100050
摘要:
本文探讨了小鼠肝脂堆积程度的超声影像学半定量检测方法。选取正常C57BL/6J小鼠、高脂饮食诱导的轻度脂肪肝HFD小鼠模型和高热能饮食诱导的中重度脂肪肝KKAy小鼠模型,分别应用肝脏病理学分析和肝组织甘油三酯含量评价动物肝脏的脂质堆积程度。用小动物超声成像系统测定肝组织内与超声波传播同方向的一定距离间2处区域的超声波回声强度,并计算回声衰减系数a值。采用 Spearman等级相关分析方法对动物肝脏甘油三酯含量和回声衰减系数a值进行相关性分析。结果显示:与CON组相比,HFD小鼠和KKAy小鼠均具有明显的肝脏脂质堆积,且KKAy小鼠的病变程度较HFD小鼠明显加重。超声波检测结果显示,与CON组相比,HFD小鼠、KKAy小鼠的回声衰减系数a值依次递增。小鼠的肝脏回声衰减系数a值与其肝脏甘油三酯含量具有明显的正相关性,相关系数r=0.744(P<0.01)。本研究表明,肝脏回声衰减系数法可以用来客观评价小鼠肝脏脂质堆积程度。该方法简便易行且具有无创性,可用于监测小鼠肝脏脂肪性病变过程及其药物治疗效果。
关键词:    脂肪性肝病      肝脏甘油三酯      超声影像学      回声衰减系数     
Establishment of an ultrasonographic method for evaluating the hepatic lipid accumulation in mice
LI Mo-han, ZHANG Xiao-lin, YE Fei
Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study; Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China
Abstract:
To investigate the semi-quantitative method for evaluating lipid accumulation in livers, male C57BL/6J mice (CON), HFD mice characterized with the mild fatty liver induced by high-fat diet, and KKAy mice charactered with the moderately severe fatty liver induced by high-caloric diet were used. The lipid accumulation was estimated by the histological examination (HE staining) and the content of hepatic triglyceride, separately. Echo-intensity of two selected regions along the ultrasound transmission direction was recorded using a small animal ultrasonographic system, and the echo-intensity attenuation coefficient was calculated. Correlation between the echo-intensity attenuation coefficient and the content of hepatic triglyceride was analyzed by the Spearman's rank correlation analysis. The results showed that the lipid accumulation in livers increased significantly in both HFD and KKAy mice compared with CON mice and it was more serious in KKAy mice than that in HFD mice. The values of echo-intensity attenuation coefficient were also increased in sequence according to group. These values were positively associated with the content of hepatic triglyceride (r=0.744, P<0.01). In conclusion, the echo-intensity attenuation coefficient is a simple, impersonal, and non-invasive method for evaluating the hepatic lipid accumulation. It can be used to research the process and the treatment of fatty liver diseases in mice.
Key words:    fatty liver disease    hepatic triglyceride    ultrasonic imaging    echo-intensity attenuation coefficient   
收稿日期: 2014-04-28
基金项目: 科技部“重大新药创制”科技重大专项(2012ZX09103-101-063;2012ZX09301-002-004)
通讯作者: 叶 菲,Tel:86-10-83150495,Fax:86-10-63017757,E-mail:yefei@imm.ac.cn
Email: yefei@imm.ac.cn
相关功能
PDF(4541KB) Free
打印本文
0
作者相关文章
李莫涵  在本刊中的所有文章
张晓琳  在本刊中的所有文章
叶菲  在本刊中的所有文章

参考文献:
[1] Hashimoto E, Tokushige K. Prevalence, gender, ethnic variations, and prognosis of NASH [J]. JGastroenterol, 2011, 46: 63-69.
[2] Yang Z, Wang X, Wen J, et al. Prevalence of non-alcoholic fatty liver disease and its relation to hypoadiponectinaemia in the middle-aged and elderly Chinese population [J]. Arch Med Sci, 2011, 7: 665-672.
[3] Hashimoto E, Taniai M, Tokushige K. Characteristics and diagnosis of NAFLD/NASH [J]. J Gastroenterol Hepatol, 2013, 28: 64-70.
[4] Saadeh S, Younossi ZM, Remer EM, et al. The utility of radiological imaging in nonalcoholic fatty liver disease [J]. Gastroenterology, 2002, 123: 745-750.
[5] The Chinese National Workshop on Fatty Liver and Alcoholic Liver Disease for the Chinese Liver Disease Association, Guidelines for management of nonalcoholic fatty liver disease: an updated and revised edition [J]. Chin J Hepatol (中华肝脏病杂志), 2010, 18: 163-166.
[6] Folch J, Lees M, Sloane-Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues [J]. J Biol Chem, 1957, 226: 497-509.
[7] Xia MF, Yan HM, He WY, et al. Standardized ultrasound hepatic/renal ratio and hepatic attenuation rate to quantify liver fat content: an improvement method [J]. Obesity, 2012, 20: 444-452.
[8] Xu YY, Hu YX. The new progression of fatty liver in integrated traditional Chinese and Western Medicine method [J]. Res Integr Tradit West Med (中西医结合研究), 2010, 2: 47-49.
[9] Cowin GJ, Jonsson JR, Bauer JD, et al. Magnetic resonance imaging and spectroscopy for monitoring liver steatosis [J]. J Magn Reson Imaging, 2008, 28: 937-945.
[10] von Volkmann HL, Havre RF, Løberg EM, et al. Quantitative measurement of ultrasound attenuation and hepato-renal index in non-alcoholic fatty liver disease [J]. Med Ultrason, 2013, 15: 16-22.
[11] Lee CH, Choi JW, Kim KA, et al. Usefulness of standard deviation on the histogram of ultrasound as a quantitative value for hepatic parenchymal echo texture; preliminary study [J]. Ultrasound Med Biol, 2006, 32: 1817-1826.
[12] Ballestri S, Lonardo A, Romagnoli D, et al. Ultrasonographic fatty liver indicator, a novel score which rules out NASH and is correlated with metabolic parameters in NAFLD [J]. Liver Int, 2012, 32: 1242-1252.
[13] Hamaguchi M, Kojima T, Itoh Y, et al. The severity of ultrasonographic findings in nonalcoholic fatty liver disease reflects the metabolic syndrome and visceral fat accumulation [J]. Am J Gastroenterol, 2007, 102: 2708-2715.
[14] Park YS, Lee CH, Choi KM, et al. Correlation between abdominal fat amount and fatty liver, using liver to kidney echo ratio on ultrasound [J]. J Korean Soc Ultrasound Med, 2012, 31: 219-224.