药学学报, 2014, 49(10): 1400-1405
引用本文:
宣荣荣, 高鑫, 吴玮, 陈海敏. 虾青素对子痫前期大鼠的预防效果[J]. 药学学报, 2014, 49(10): 1400-1405.
XUAN Rong-rong, GAO Xin, WU Wei, CHEN Hai-min. Effect of astaxanthin on preeclampsia rat model[J]. Acta Pharmaceutica Sinica, 2014, 49(10): 1400-1405.

虾青素对子痫前期大鼠的预防效果
宣荣荣1, 高鑫2, 吴玮2, 陈海敏2
1. 宁波大学医学院附属医院, 浙江 宁波 315020;
2. 宁波大学应用海洋生物技术教育部重点实验室, 浙江 宁波 315211
摘要:
探讨虾青素对防治L-亚硝酸左旋精氨酸甲酯(L-NAME)诱导的子痫前期大鼠模型的效果及其机制。采用L-NAME诱导建立子痫前期大鼠模型,在妊娠第5~20天,虾青素组灌胃25 mg·kg-1·d-1·bw-1虾青素;于妊娠第10~20天,L-NAME建模组和虾青素组皮下注射L-NAME 125 mg·kg-1·d-1·bw-1。检测大鼠的血压、尿蛋白;测定血清中MDA、SOD、NOS、NO和内皮素;HE染色分析胎盘组织病变;免疫组化分析NF-κB(nuclear factor kappa B)、ROCK Ⅱ(Rho-associated protein kinase Ⅱ)、HO-1(heme oxygenase)和Caspase3的表达。结果显示,L-NAME建模组产生典型的子痫前期症状;血液中MDA、内皮素增高,NOS、SOD下降等。而经虾青素处理后,血压显著降低,尿蛋白减少,血清中MDA显著下降,SOD明显增加(P<0.05),NO升高,病理现象改善。免疫组化结果显示,虾青素组中的NF-κB、Caspase3(P<0.05)及ROCK Ⅱ的表达降低、HO-1的表达增加。本研究显示,虾青素有助于减少子痫前期大鼠胎盘的氧化应激状态,从而有效改善子痫前期孕鼠的高血压、高尿蛋白等病理反应。
关键词:    虾青素      L-亚硝酸左旋精氨酸甲酯      子痫前期      氧化应激     
Effect of astaxanthin on preeclampsia rat model
XUAN Rong-rong1, GAO Xin2, WU Wei2, CHEN Hai-min2
1. The Affiliated Hospital of Medical College of Ningbo University, Ningbo 315020, China;
2. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China
Abstract:
The effect of astaxanthin on Nω-nitro-L-arginine methyl ester (L-NAME) induced preeclampsia disease rats was investigated. Thirty pregnant Spraque-Dawley rats were randomly divided into three groups (n=10): blank group, L-NAME group and astaxanthin group. From day 5 to 20, astaxanthin group rats were treated with astaxanthin (25 mg·kg-1·d-1·bw-1) from pregnancy (day 5). To establish the preeclamptic rat model, L-NAME group and astaxanthin group rats were injected with L-NAME (125 mg·kg-1·d-1·bw-1) from days 10-20 of pregnancy. The blood pressure and urine protein were recorded. Serum of each group was collected and malondialdehyde (MDA), superoxide dismutase (SOD) and nitric oxide synthase (NOS) activities were analyzed. Pathological changes were observed with HE stain. The expression of NF-κB (nuclear factor kappa B), ROCK Ⅱ (Rho-associated protein kinase II), HO-1 (heme oxygenase-1) and Caspase 3 were analyzed with immunohistochemistry. L-NAME induced typical preeclampsia symptoms, such as the increased blood pressure, urinary protein, the content of MDA, etc. Astaxanthin significantly reduced the blood pressure (P<0.01), the content of MDA (P<0.05), and increased the activity of SOD (P<0.05) of preeclampsia rats. The urinary protein, NO, and NOS were also decreased. HE stain revealed that after treated with astaxanthin, the thickness of basilal membrane was improved and the content of trophoblast cells and spiral arteries was reduced. Immunohistochemistry results revealed that the expressions of NF-κB, ROCK Ⅱ and Caspase 3 in placenta tissue were effectively decreased, and HO-1 was increased. Results indicated that astaxanthin can improve the preeclampsia symptoms by effectively reducing the oxidative stress and inflammatory damages of preeclampsia. It revealed that astaxanthin may be benefit for prevention and treatment of preeclampsia disease.
Key words:    astaxanthin    L-NAME    preeclampsia    oxidative stress   
收稿日期: 2014-05-14
基金项目: 国家自然科学基金资助项目(81370532);浙江省医药卫生科技计划项目(2013KYB238);宁波市自然科学基金资助项目(2013A610220)
通讯作者: 陈海敏,Tel/Fax:86-574-87609574,E-mail:chenhaimin@nbu.edu.cn
Email: chenhaimin@nbu.edu.cn
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