药学学报, 2014, 49(10): 1446-1450
引用本文:
贺晶, 李艳, 司南, 赵海誉, 边宝林, 王宏洁. 应用UPLC-Orbitrap质谱分析鲜蟾酥与干蟾酥化学成分差异[J]. 药学学报, 2014, 49(10): 1446-1450.
HE Jing, LI Yan, SI Nan, ZHAO Hai-yu, BIAN Bao-lin, WANG Hong-jie. Comparison of the chemical composition between fresh and dried Venenum Bufonis by UPLC-Orbitrap MS[J]. Acta Pharmaceutica Sinica, 2014, 49(10): 1446-1450.

应用UPLC-Orbitrap质谱分析鲜蟾酥与干蟾酥化学成分差异
贺晶, 李艳, 司南, 赵海誉, 边宝林, 王宏洁
中国中医科学院中药研究所, 北京 100700
摘要:
为阐明鲜蟾酥的化学成分以及鲜蟾酥与干蟾酥化学成分差异,本研究对黑眶蟾蜍的新鲜耳后腺分泌液及干蟾酥中的化学成分进行了比较研究。采用超高效液相色谱串联静电场轨道阱高分辨质谱(UPLC-Orbitrap MS)技术,以乙腈和水(含体积比0.1% 甲酸)为流动相对其进行梯度洗脱,并在高分辨质谱正离子在线检测模式下,通过比对化合物的精确分子量及质谱碎片信息对其中39个主要色谱峰进行了鉴定。鲜蟾酥及干蟾酥主要成分的化学模式存在较大差别,与干蟾酥相比,鲜蟾酥在蟾毒配基类成分上的含量低、种类少。
关键词:    鲜蟾酥      化学成分      UPLC-Orbitrap MS     
Comparison of the chemical composition between fresh and dried Venenum Bufonis by UPLC-Orbitrap MS
HE Jing, LI Yan, SI Nan, ZHAO Hai-yu, BIAN Bao-lin, WANG Hong-jie
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
Abstract:
To identify the active components in Bufo melanostictus Schneider and clarify the difference between fresh and dried Venenum Bufonis, a UPLC-Orbitrap MS method has been established. The separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. By comparing their retention time and high resolution mass data of Venenum Bufonis extracts, 39 effective components were primarily identified by MS/MS analysis in positive ion mode. Twenty-six of them were bufadienolides. There were significant differences in the main composition between fresh and dried Venenum Bufonis. There are fewer bufadienolides in fresh toad venom.
Key words:    toad venum    chemical profile    UPLC-Orbitrap MS   
收稿日期: 2014-04-18
基金项目: 科技部重大新药创制专项(2013zx09102025);中国中医科学院自选课题(z02063)
通讯作者: 王宏洁,Tel/Fax:86-10-64021008,E-mail:wang66397@sina.com
Email: wang66397@sina.com
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