药学学报, 2014, 49(10): 1451-1446
引用本文:
童菊华, 王玉霞, 孙虹, 廖娜, 李先恩, 秦民坚, 庞小存. 基于代谢组学技术的杭麦冬和川麦冬药效差异性研究[J]. 药学学报, 2014, 49(10): 1451-1446.
TONG J�-hua, WANG Yu-xia, SUN Hong, LIAO Na, LI Xian-en, QIN Min-jian, PANG Xiao-cun. Pharmacodynamic differences between Hangmaidong and Chuanmaidong based on metabonomics[J]. Acta Pharmaceutica Sinica, 2014, 49(10): 1451-1446.

基于代谢组学技术的杭麦冬和川麦冬药效差异性研究
童菊华1,2, 王玉霞1, 孙虹1, 廖娜2, 李先恩1, 秦民坚2, 庞小存1
1. 中国医学科学院、北京协和医学院药用植物研究所, 北京 100193;
2. 中国药科大学中药学院, 江苏 南京 210009
摘要:
运用超高效液相-四级杆飞行时间质谱(UPLC-Q-TOF/MS)结合多变量统计学分析,探讨利用代谢组学的研究方法比较杭麦冬和川麦冬对正常大鼠血浆内源性代谢物干预作用的差异性。结果显示川麦冬低、中剂量组与对照组相近,高剂量组则有明显区别;杭麦冬高、中、低剂量组与对照组明显不同,剂量越大距离越远;具有显著性差异的内源性代谢产物为溶血磷脂酰胆碱类物质,与心血管系统类疾病紧密相关。与川麦冬给药组相比,杭麦冬组对正常大鼠血浆内源性代谢产物的干预作用更显著。从药效学上看,杭麦冬和川麦冬差异显著。
关键词:    杭麦冬      川麦冬      药效学差异性      代谢组学      UPLC-Q-TOF/MS     
Pharmacodynamic differences between Hangmaidong and Chuanmaidong based on metabonomics
TONG J�-hua1,2, WANG Yu-xia1, SUN Hong1, LIAO Na2, LI Xian-en1, QIN Min-jian2, PANG Xiao-cun1
1. Institute of Medical Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;
2. School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, China
Abstract:
To evaluate the differences of Ophiopogon japonicus from different cultivations, the metabolomics based method was conducted to compare the effects of Hangmaidong and Chuanmaidong (Chinese name) on plasma endogenous metabolites of normal rats. Data were collected by ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS), and were analyzed by multivariate statistical method, such as Principal Component Analysis and Orthogonal Signal Correction Partial Least Square Discriminant Analysis. Results revealed that the plasma metabolites profiling of low and middle dose group of Chuanmaidong were similar to the control group, but different from the high dose group obviously. Meanwhile the high, middle and low dose groups of Hangmaidong were different from control group notably, and the difference is dose dependent. Lysophosphatidylcholines, the possible endogenous metabolites which contribute to the classification most significantly, are closely related to cardiovascular system diseases. Compared with the group of Chuanmaidong, Hangmaidong has greater impact on the plasma metabolic profiling of normal rats. Hangmaidong and Chuanmaidong showed significant differences pharmacodynamically.
Key words:    Hangmaidong    Chuanmaidong    pharmacodynamic difference    metabonomics    UPLC-Q-TOF/MS   
收稿日期: 2014-04-09
基金项目: 国家自然科学基金资助项目(81130069)
通讯作者: 李先恩,Tel:86-10-62810019,E-mail:xeli@implad.ac.cn;秦民坚,Tel:86-25-85391290,E-mail:minjianqin@sina.com
Email: xeli@implad.ac.cn;minjianqin@sina.com
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