药学学报, 2014, 49(12): 1730-1738
引用本文:
郑司浩, 李亚康, 任伟光, 黄林芳. 基于DNA条形码的百合属分子鉴定[J]. 药学学报, 2014, 49(12): 1730-1738.
ZHENG Si-hao, LI Ya-kang, REN Wei-guang, HUANG Lin-fang. Molecular identification in genus of Lilium based on DNA barcoding[J]. Acta Pharmaceutica Sinica, 2014, 49(12): 1730-1738.

基于DNA条形码的百合属分子鉴定
郑司浩, 李亚康, 任伟光, 黄林芳
中国医学科学院、北京协和医学院药用植物研究所, 北京 100193
摘要:
本文通过实验, 并结合GenBank数据库序列, 分析ITS、ITS2、psbA-trnH、matK、rbcL 5条常用条形码共978条序列在种内与种间变异、barcoding gap、系统发育树等方面对百合属植物的鉴别能力, 并采用最近距离法 (nearest distance) 和相似性搜索算法 (BLAST1) 评价5条序列的鉴定成功率.研究结果显示, 不同条形码序列可以较好的鉴别百合属内不同物种.ITS序列鉴定成功率较高, 种内种间差异显著, 系统发育树可以很好地将不同物种分开.结果表明DNA条形码技术可以准确地鉴别百合属植物, ITS序列可以作为鉴别百合属不同物种的优选序列.
关键词:    百合属      DNA条形码      分子鉴别      ITS     
Molecular identification in genus of Lilium based on DNA barcoding
ZHENG Si-hao, LI Ya-kang, REN Wei-guang, HUANG Lin-fang
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
Abstract:
To establish a new method for identifying genus of Lilium by DNA barcoding technology, ITS, ITS2, psbA-trnH, matK and rbcL sequences were analyzed in term of variation of inter-and intra-species, barcoding gap, neighbor-joining tree to distinguish genus of Lilium based on 978 sequences from experimental and GenBank database, and identification efficiency was evaluated by Nearest distance and BLAST1 methods. The results showed that DNA barcoding could identify different species in genus of Lilium. ITS sequence performed higher identification efficiency, and had significant difference between intra-and inter-species. And NJ tree could also divide species into different clades. Results indicate that DNA barcoding can identify genus of Lilium accurately. ITS sequence can be the optimal barcode to identify species of Lilium.
Key words:    Lilium    DNA barcoding    molecular identification    ITS   
收稿日期: 2014-07-16
基金项目: 国家自然科学基金资助项目(81274013,81473315);国家自然科学基金重点项目(81130069)
通讯作者: 黄林芳
Email: lfhuang@implad.ac.cn
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