药学学报, 2014, 49(12): 1699-1704
引用本文:
王璐, 丁家昱, 刘秀秀, 汤明海, 晁若冰, 王锋鹏. 附子中胺醇型二萜生物碱的鉴定及其强心活性研究[J]. 药学学报, 2014, 49(12): 1699-1704.
WANG Lu, DING Jia-yu, LIU Xiu-xiu, TANG Ming-hai, CHAO Ruo-bing, WANG Feng-peng. Identification of aminoalcohol-diterpenoid alkaloids in Aconiti Lateralis Radix Praeparata and study of their cardiac effects[J]. Acta Pharmaceutica Sinica, 2014, 49(12): 1699-1704.

附子中胺醇型二萜生物碱的鉴定及其强心活性研究
王璐1, 丁家昱1, 刘秀秀1, 汤明海2, 晁若冰1, 王锋鹏1
1. 四川大学, 华西药学院, 四川 成都 610041;
2. 四川大学, 生物治疗国家重点实验室, 四川 成都 610041
摘要:
为确定附子中的强心有效成分, 本文对附子中的胺醇型二萜生物碱进行了系统研究.采用超高效液相色谱与电喷雾质谱联用技术分离了附子中的41种成分, 通过与对照品的保留时间和质谱数据进行对比鉴定了13种胺醇型二萜生物碱, 分别是北乌宁、中乌宁、多根乌头碱、异塔拉定、乌头原碱、8-甲氧基中乌宁、次乌宁、附子灵、3-去氧乌头原碱、尼奥灵、8-甲氧基次乌宁、塔拉萨敏、查斯曼宁.同时, 测定了7批不同产地和炮制方法的附子, 样品中所含化学成分的种类相似但含量差异较大.此外, 利用离体蛙心灌流实验对这13种胺醇型二萜生物碱进行了强心活性实验, 结果显示6种成分具有强心作用.实验结果表明胺醇型二萜生物碱是附子中的强心活性成分, 本研究对附子中胺醇型二萜生物碱及强心作用的研究具有重要的参考意义.
关键词:    附子      胺醇型二萜生物碱      UPLC-ESI-MS      强心作用     
Identification of aminoalcohol-diterpenoid alkaloids in Aconiti Lateralis Radix Praeparata and study of their cardiac effects
WANG Lu1, DING Jia-yu1, LIU Xiu-xiu1, TANG Ming-hai2, CHAO Ruo-bing1, WANG Feng-peng1
1. West China School of Pharmacy, Chengdu 610041, China;
2. State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China
Abstract:
In order to affirm the cardioactive components in Fuzi, we identified a group of aminoalcohol-diterpenoid alkaloids in Fuzi using ultra high-performance liquid chromatography coupled with electrospray ionization mass spectrometer (UPLC-ESI-MS) method. Among a total of forty-one isolated ingredients, thirteen major aminoalcohol-diterpenoid alkaloids were identified by comparing their retention times and MS spectra with those of the reference substances. Moreover, Fuzi samples from different places of origin and with different processing methods were examined and their components displayed a pattern of high similarity, though the relative abundance varies probably due to their different processing methods. Furthermore, the cardiac effect of each identified alkaloid was individually evaluated using the isolated bullfrog heart perfusion experiment. Among the thirteen aminoalcohol-diterpenoid alkaloids tested, six of them significantly enhanced the amplitude rates. Taken together, we affirm that the cardioactive components in Fuzi are aminoalcohol-diterpenoid alkaloids, shedding light on future studies of the mechanisms and development of these cardioactive compounds.
Key words:    Aconiti Lateralis Radix Praeparata    aminoalcohol-diterpenoid alkaloids    UPLC-ESI-MS    cardiac effect   
收稿日期: 2014-06-16
通讯作者: 刘秀秀, 汤明海
Email: liuxiuxiu@scu.edu.cn;tangminghai1982@126.com
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