药学学报, 2015, 50(7): 830-835
引用本文:
季涛, 张丽丽, 黄晓晨, 宿树兰, 欧阳臻, 朱振华, 郭盛, 尚尔鑫, 钱大玮, 段金廒. 基于代谢组学的桑叶多组分治疗糖尿病的作用机制研究[J]. 药学学报, 2015, 50(7): 830-835.
JI Tao, ZHANG Li-li, HUANG Xiao-chen, SU Shu-lan, OUYANG Zhen, ZHU Zhen-hua, GUO Sheng, SHANG Er-xin, QIAN Da-wei, DUAN Jin-ao. The action mechanisms of Morus alba leaves extract for the treatment of diabetes based on plasma metabolomics[J]. Acta Pharmaceutica Sinica, 2015, 50(7): 830-835.

基于代谢组学的桑叶多组分治疗糖尿病的作用机制研究
季涛1,2, 张丽丽1,2, 黄晓晨1,2, 宿树兰1,2, 欧阳臻3, 朱振华1,2, 郭盛1,2, 尚尔鑫1,2, 钱大玮1,2, 段金廒1,2
1. 南京中医药大学 江苏省中药资源产业化过程协同创新中心, 中药资源产业化与方剂创新药物国家地方联合工程 研究中心, 江苏 南京 210023;
2. 南京中医药大学 江苏省方剂高技术研究重点实验室, 江苏 南京 210023;
3. 江苏大学药学院, 江苏 镇江 212013
摘要:
采用超高效液相色谱串联静电场轨道阱高分辨质谱 (UPLC-OrbitrapMS) 技术结合多变量统计学分析, 利用代谢组学的研究方法和技术探讨糖尿病模型小鼠血浆整体代谢物谱特征、代谢轮廓变化和生物标志物群, 并对桑叶生物碱类、黄酮类、多糖类提取物干预后的生物效应转归与分子机制进行研究。通过内源性代谢物的一级及二级碎片离子的精确分子量信息, 鉴定了糖尿病模型小鼠血浆中8个潜在生物标志物, 主要涉及鞘脂、氨基酸代谢途径。主成分分析表明, 正常组与糖尿病模型组得到明显区分, 提示模型小鼠体内代谢发生异常; 桑叶生物碱类、黄酮类、多糖类提取物干预后对糖尿病模型小鼠均有不同程度的回调作用, 其作用机制与其调节氨基酸代谢、鞘脂代谢密切相关。
关键词:    桑叶      糖尿病      UPLC-Orbitrap MS      代谢组学      作用机制     
The action mechanisms of Morus alba leaves extract for the treatment of diabetes based on plasma metabolomics
JI Tao1,2, ZHANG Li-li1,2, HUANG Xiao-chen1,2, SU Shu-lan1,2, OUYANG Zhen3, ZHU Zhen-hua1,2, GUO Sheng1,2, SHANG Er-xin1,2, QIAN Da-wei1,2, DUAN Jin-ao1,2
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing 210023, China;
2. Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China;
3. School of Pharmacy, Jiangsu University, Zhenjiang 212013, China
Abstract:
In order to evaluate the effect and mechanism of the mulberry leaf alkaloid, flavones, and polysaccharide intervention on diabetes, the overall metabolite profiling characteristics for the plasma of diabetic mouse was performed by using an ultra-performance liquid chromatography/electrospray-tandem mass spectrometry (UPLC-ESI-MS). The 8 potential biomarkers were found in diabetic mice plasma based on the data of MS/MS characteristics obtained from the UPLC-OrbitrapMS analysis, which mainly involved in sphingolipids, amino acid metabolic pathway. The principal component analysis showed that the normal group and model group were obviously distinguished and implied that metabolic disturbance was happened in diabetic mice plasma. The extracts of mulberry leaf flavonoids, polysaccharide, alkaloid had exhibited the effects of callback function for diabetic mice through regulating the amino acid metabolism and sphingolipid metabolism.
Key words:    mulberry leaf    diabetes    UPLC-Orbitrap MS    metabonomics    action mechanism   
收稿日期: 2014-11-15
基金项目: 国家自然科学基金资助项目 (81373889, 30973885); 教育部新世纪优秀人才支持计划项目 (NCET-13-0873); 江苏省"六大人才高峰"项目 (YY-010); 江苏省"333高层次人才培养工程"项目 (2013).
通讯作者: 宿树兰, 段金廒
Email: sushulan@njutcm.edu.cn;dja@njutcm.edu.cn
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