药学学报, 2015, 50(7): 882-886
引用本文:
曾平燕, 张宇, 芮雯, 吴霞, 冯毅凡. 2型糖尿病模型大鼠血浆磷脂的UPLC-Q-TOF/MS分析[J]. 药学学报, 2015, 50(7): 882-886.
ZENG Ping-yan, ZHANG Yu, RUI Wen, WU Xia, FENG Yi-fan. UPLC-Q-TOF/MS analysis of phospholipids metabolite profiling in plasma of type 2 diabetes mellitus rat[J]. Acta Pharmaceutica Sinica, 2015, 50(7): 882-886.

2型糖尿病模型大鼠血浆磷脂的UPLC-Q-TOF/MS分析
曾平燕, 张宇, 芮雯, 吴霞, 冯毅凡
广东药学院中心实验室, 广东 广州 510006
摘要:
对2型糖尿病 (T2DM) 模型大鼠与正常组大鼠血浆磷脂代谢的情况进行对比分析, 寻找相关的病理生物标记物。将SD大鼠随机分为2组: 正常对照组 (NC) 和2型糖尿病模型组 (MC), 采用UPLC-Q-TOF/MS研究机体在正常状态、2型糖尿病状态下的大鼠体内血浆脂质代谢物变化的情况。得到大量的数据, 结合二级质谱裂解数据、元素组成、数据库匹配, 鉴定得到大鼠血浆磷脂成分, 再通过R软件中BioMark软件包实现对两组样品中特异性的标志物进行筛选。筛选出的磷脂病理标记物主要为磷脂酰胆碱类 (PC) 和甘油三酯类 (TG), 其中MC组中大鼠血浆二酰基PC类的含量明显低于NC组大鼠, 在病理状态下表现为高碳数高不饱和度的TG类成分的减少。在2型糖尿病状态下, 大鼠血浆磷脂发生了明显的代谢变化, 而这些代谢变化与2型糖尿病的发生与发展密切相关。
关键词:    2型糖尿病      磷脂代谢      生物标记物     
UPLC-Q-TOF/MS analysis of phospholipids metabolite profiling in plasma of type 2 diabetes mellitus rat
ZENG Ping-yan, ZHANG Yu, RUI Wen, WU Xia, FENG Yi-fan
The Central Laboratory of Guangdong Pharmaceutical University, Guangzhou 510006, China
Abstract:
This study reported the analysis of plasma phospholipid metabolism of the rats and the pathological biomarkers between the type 2 diabetes model control group (MC) and the normal control group (NC). SD rats were randomly divided into 2 groups: NC and MC. To investigate state of plasma metabolite profiling in normal body, type 2 diabetes mellitus (T2DM) model group using UPLC-Q-TOF/MS which was used as analysis tool in this research. The compounds were identified by UPLC-Q-TOF/MS based on MS/MS fragment ions information, element composition in MassLynx 4.1 and the Lipid Maps database. The sign of two groups of samples in specific markers for screening was through a software package in R software (BioMark software). The results show that the pathological markers were mainly phosphatidylcholine (PC) and triglycerides (TG); the 2-acyl PC in the MC group was less more obviously than that in the NC group; high carbon number and high degree of unsaturation of the TG was reduced under the condition of type 2 diabetes. In the state of type 2 diabetes, metabolic changes occurred in rat plasma phospholipids obviously, which had a close relationship with the occurrence and development of T2DM.
Key words:    type 2 diabetes    phospholipid metabolism    biomarker   
收稿日期: 2014-12-25
基金项目: 广东省重大科技专项资金资助项目 (2013A022100040).
通讯作者: 吴霞, 冯毅凡
Email: wxiaxia@163.com;yffeng@139.com
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