药学学报, 2015, 50(7): 906-909
引用本文:
臧艺玫, 李妍芃, 乔晶, 陈宏昊, 刘春生. 基于β-香树脂醇合成酶基因SNP的甘草道地性机制研究[J]. 药学学报, 2015, 50(7): 906-909.
ZANG Yi-mei, LI Yan-peng, QIAO Jing, CHEN Hong-hao, LIU Chun-sheng. Mechanism of genuineness of Glycyrrhiza uralensis based on SNP of β-Amyrin synthase gene[J]. Acta Pharmaceutica Sinica, 2015, 50(7): 906-909.

基于β-香树脂醇合成酶基因SNP的甘草道地性机制研究
臧艺玫, 李妍芃, 乔晶, 陈宏昊, 刘春生
北京中医药大学中药学院, 北京 100102
摘要:
本文采用PCR-SSCP和测序组合技术检测了6个产地甘草的β-香树脂醇合成酶 (β-AS) 基因的SNP, 并进行了SNP和产地的相关性分析, 探索甘草道地性形成的分子机制。结果显示, 甘草β-AS基因第一外显子存在1处SNP, 可将甘草划分为94A型、94G型和94A/G型3种基因型, 其中94A型在道地产区所占比例要远远高于非道地产区, 达到极显著差异 (P < 0.001)。本文研究证明, 甘草β-AS基因94位点的SNP可能是导致甘草道地性形成的机制之一。
关键词:    甘草      道地性      β-香树脂醇合成酶基因      单核苷酸多态性     
Mechanism of genuineness of Glycyrrhiza uralensis based on SNP of β-Amyrin synthase gene
ZANG Yi-mei, LI Yan-peng, QIAO Jing, CHEN Hong-hao, LIU Chun-sheng
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China
Abstract:
β-Amyrin synthase (β-AS) genes of Glycyrrhiza uralensis from 6 different regions were analyzed by PCR-SSCP and sequenced, then the correlationship between β-AS SNP and regions of Glycyrrhiza uralensis were determined. According to the 1 coding single nucleotide polymorphism on the first exon of β-AS gene at 94 bp site, Glycyrrhiza uralensis could be divided into 3 genotypes. In these genotypes, the percentage of 94A type in genuine regions was much higher, and it had significant differences with the percentage in non-genuine regions (P < 0.001). The results of the experiment proved that different β-AS genotypes at 94 bp site from different regions may be one of the important reasons to result in the genuineness of Glycyrrhiza uralensis.
Key words:    Glycyrrhiza uralensis    genuineness    β-amyrin synthase gene    single nucleotide polymorphisms   
收稿日期: 2015-02-02
基金项目: 国家自然科学基金资助项目 (539/03517); 博士点基金资助项目 (537/03010).
通讯作者: 刘春生
Email: max_liucs@263.net
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