药学学报, 2016, 51(1): 153-156
引用本文:
黄祥涛, 肖润梅, 王明凤, 王俊俊, 陈勇. 槟榔碱对大鼠肝脏CYP2E1的体内诱导作用[J]. 药学学报, 2016, 51(1): 153-156.
HUANG Xiang-tao, XIAO Run-mei, WANG Ming-feng, WANG Jun-jun, CHEN Yong. Induction of rat hepatic CYP2E1 expression by arecoline in vivo[J]. Acta Pharmaceutica Sinica, 2016, 51(1): 153-156.

槟榔碱对大鼠肝脏CYP2E1的体内诱导作用
黄祥涛, 肖润梅, 王明凤, 王俊俊, 陈勇
湖北大学中药生物技术省重点实验室, 湖北大学生物资源绿色转化协同创新中心, 湖北 武汉 430062
摘要:
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Induction of rat hepatic CYP2E1 expression by arecoline in vivo
HUANG Xiang-tao, XIAO Run-mei, WANG Ming-feng, WANG Jun-jun, CHEN Yong
Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan 430062, China
Abstract:
The regulation mechanism of arecoline on rat hepatic CYP2E1 was studied in vivo. After oral administration of arecoline hydrobromide (AH; 4, 20 and 100 mg·kg-1·d-1) to rats for one week, the hepatic CYP2E1 mRNA level remained unchanged, but the hepatic CYP2E1 protein content was dose-dependently increased. Additionally, although the hepatic CYP2E1 activity was induced by AH treatment, the induction was attenuated with the increase in dosage. The results indicate that the effect of arecoline on rat hepatic CYP2El does not involve transcriptional activation of the gene, but largely involves the stabilization of CYP2E1 protein against degradation or increased efficiency of CYP2E1 mRNA translation, and additionally involve the post- translational modification of CYP2E1 protein. Furthermore, the CYP2E1 response is fairly equal among the different species, the induction of rat hepatic CYP2E1 by arecoline suggests that there is a risk of metabolic interaction among the substrate drugs of CYP2E1 in betel-quid use human.
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收稿日期: 2015-05-25
DOI: 10.16438/j.0513-4870.2015-0488
通讯作者: 陈勇
Email: cy101610@qq.com
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