药学学报, 2016, 51(3): 338-346
引用本文:
杨盛, 何然, 张飞燕, 薛强, 徐晓玉. 细胞共培养模型及其在中枢神经系统疾病研究中的应用[J]. 药学学报, 2016, 51(3): 338-346.
YANG Sheng, HE Ran, ZHANG Fei-yan, XUE Qiang, XU Xiao-yu. Application of cell co-culture techniques in central nervous system diseases[J]. Acta Pharmaceutica Sinica, 2016, 51(3): 338-346.

细胞共培养模型及其在中枢神经系统疾病研究中的应用
杨盛, 何然, 张飞燕, 薛强, 徐晓玉
西南大学药学院 & 中医药学院, 重庆 400715
摘要:
中枢神经系统疾病的深入研究离不开神经组织细胞的体外培养。神经组织细胞的单独培养难以反映在体细胞间的相互影响,而其共培养模型可以最大程度地模拟体内环境,观察细胞与细胞、细胞与胞外环境之间的相互作用,被越来越广泛地用于诸如脑卒中、帕金森病、阿尔茨海默病和肌萎缩侧索硬化症等中枢神经系统疾病的体外研究。"神经血管单元"是由大脑神经元、脑微血管内皮细胞和星形胶质细胞为主组成的体现大脑结构和功能的基本单位,其体外模型的建立对研究中枢神经系统疾病具有重要意义。本文结合细胞间接触和非接触的培养方式,从二维和三维培养的技术角度出发,概述中枢神经组织细胞的共培养模型及其在相关疾病研究中的应用,并展望其在未来研究中的发展方向。
关键词:    脑卒中      帕金森病      阿尔茨海默病      肌萎缩侧索硬化症      细胞共培养      神经血管单元     
Application of cell co-culture techniques in central nervous system diseases
YANG Sheng, HE Ran, ZHANG Fei-yan, XUE Qiang, XU Xiao-yu
College of Pharmaceutical Sciences and College of Chinese Medicine, Southwest University, Chongqing 400715, China
Abstract:
The study of central nervous system disease is dependent on in vitro culture of neuronal cells. However, it is hard to determine the interaction between cells in culture of single type of neuronal cells. The co-culture system is able to mimic the cell-cell interaction in the brain and to facilitate investigation into the interaction between different types of cells, as well as cell-environment interaction. The co-culture of neurocytes is more and more popular in the disease study of central nervous system in vitro, such as stroke, Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, etc. Neurovascular unit (NVU), which is composed of neurons, brain microvascular endothelial cells and astrocytes, can reflect the structure and function of brain in state of the art. Establishment of NVU in vitro is important in the study of the brain diseases. In this paper, several co-culture models of the central nervous system are reviewed in techniques for two-dimensional and three-dimensional culturing. Cell contact and non-contact methods are compared. Moreover, their application in the relevant research and the future direction are explored.
Key words:    stroke    Parkinson's disease    Alzheimer's disease    amyotrophic lateral sclerosis    co-culture of cells    neurovascular unit   
收稿日期: 2015-07-01
DOI: 10.16438/j.0513-4870.2015-0583
基金项目: 国家自然科学基金面上项目(81473549);国家科技重大新药创制专项(2014ZX09304-306-04);教育部中央高校基本科研业务费(XDJK2014D023).
通讯作者: 徐晓玉
Email: xuxiaoyu@swu.edu.cn
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