药学学报, 2016, 51(6): 972-978
引用本文:
李秀杨, 段素敏, 刘欢欢, 徐金娣, 孔铭, 刘红权, 李松林. 特征含硫衍生物对硫熏牡丹皮检控的意义[J]. 药学学报, 2016, 51(6): 972-978.
LI Xiu-yang, DUAN Su-min, LIU Huan-huan, XU Jin-di, KONG Ming, LIU Hong-quan, LI Song-lin. Sulfur-containing derivatives as characteristic chemical markers in control of sulfur-fumigated Moutan Cortex[J]. Acta Pharmaceutica Sinica, 2016, 51(6): 972-978.

特征含硫衍生物对硫熏牡丹皮检控的意义
李秀杨1,2, 段素敏2, 刘欢欢1,2, 徐金娣1,2, 孔铭1,2, 刘红权3, 李松林1,2
1. 南京中医药大学附属中西医结合医院中药质量研究室, 江苏 南京 210028;
2. 江苏省中医药研究院中药代谢组研究室, 江苏 南京 210028;
3. 南京中医药大学附属中西医结合医院神经内科, 江苏 南京 210028
摘要:
二氧化硫残留量是当前硫熏药材检控的法定指标,但该指标能否客观反映药材硫熏程度有待深入研究。牡丹皮硫熏后会产生特征含硫衍生物。本文采用UPLC-QTOF-MS/MS对硫熏牡丹皮中特征含硫衍生物进行确证,并对贮藏前后特征含硫衍生物的量与二氧化硫残留量进行统计学分析。发现贮藏可导致药材中二氧化硫残留量显著下降,但特征含硫衍生物含量没有明显改变,说明二氧化硫残留量不一定能真正反映牡丹皮的硫熏程度,以特征含硫衍生物作为硫熏药材的检控指标更具适用性。
关键词:    牡丹皮      硫熏      二氧化硫残留      特征含硫衍生物      UPLC-QTOF-MS/MS     
Sulfur-containing derivatives as characteristic chemical markers in control of sulfur-fumigated Moutan Cortex
LI Xiu-yang1,2, DUAN Su-min2, LIU Huan-huan1,2, XU Jin-di1,2, KONG Ming1,2, LIU Hong-quan3, LI Song-lin1,2
1. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nangjing 210028, China;
2. Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine, Nangjing 210028, China;
3. Department of Neurology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nangjing 210028, China
Abstract:
The amount of sulfur dioxide residue is currently employed by Chinese Pharmacopoeia (CP) as an index to screen sulfur-fumigated herbs, but it is unclear if this index can objectively reflect the quality of sulfur-fumigated herbs. In the present study, sulfur-containing derivatives were confirmed in sulfur-fumigated Moutan Cortex (MC) by UPLC-QTOF-MS/MS analysis, and the contents of sulfur-containing derivatives and sulfur dioxide residues were statistically analyzed both in self-made and commercially available sulfur-fumigated and non-fumigated MC as well as the samples thereof before and after eight-month storage. The amount of sulfur dioxide was significantly decreased, but that of the newly-generated sulfur-containing markers was not, after eight-month storage of the sulfur-fumigated MC samples, indicating that the amount of sulfur dioxide residue may not be positively correlated with the quality of sulfur-fumigated MC. Therefore, sulfur dioxide residue index alone may not objectively reflect the sulfur-fumigation extent (quality change extent) of MC, more specific method using characteristic sulfur-containing derivatives as chemical markers should be developed to supplement the sulfur dioxide residue determination in the quality control of sulfur-fumigated MC.
Key words:    Moutan Cortex    sulfur-fumigation    sulfur dioxide residue    sulfur-containing derivative    UPLCQTOF- MS/MS   
收稿日期: 2015-12-07
DOI: 10.16438/j.0513-4870.2015-1120
基金项目: 国家高技术研究发展计划(863计划)资助项目(2014AA022204);国家自然科学基金资助项目(81503245);江苏省中医药管理局项目(LZ13075);江苏省六大人才高峰项目(YY-007);中国中医科学院江苏分院院级课题(JSBN1301).
通讯作者: 李松林,Tel/Fax:86-25-85639640,E-mail:songlinli64@126.com
Email: songlinli64@126.com
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参考文献:
[1] Li SL, Shen H, Zhu LY, et al. Ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry based chemical profiling approach to rapidly reveal chemical transformation of sulfur-fumigated medicinal herbs, a case study on white ginseng[J]. J Chromatogr A, 2012, 1231:31-45.
[2] Jin X, Zhu LY, Shen H, et al. Influence of sulfur-fumigation on the quality of white ginseng:a quantitative evaluation of major ginsenosides by high performance liquid chromatography[J]. Food Chem, 2012, 135:1141-1147.
[3] Li ZY, Gao HM, Sun J, et al. Secoiridoid sulfonates from the sulfiting-processed buds of Lonicera japonica[J]. Helv Chim Acta, 2012, 95:1144-1151.
[4] Wang Q, Liu R, Guo H, et al. Simultaneous LC determination of major constituents in red and white peony root[J]. Chromatographia, 2005, 62:581-588.
[5] Hayes PY, Lehmann R, Penman K, et al. Sodium paeoniflorin sulfonate, a process derived artifact from paeoniflorin[J]. Tetrahedron Lett, 2005, 46:2615-2618.
[6] Li SL, Song JZ, Choi F, et al. Chemical profiling of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight mass spectrometry[J]. J Pharm Biomed Anal, 2009, 49:253-266.
[7] Duan SM, Xu J, Bai YJ, et al. Sulfur dioxide residue in sulfur-fumigated edible herbs:the fewer, the safer?[J]. Food Chem, 2016, 192:119-124.
[8] Cheng Y, Peng C, Wen F, et al. Pharmacokinetic comparisons of typical constituents in white peony root and sulfur fumigated white peony root after oral administration to mice[J]. J Ethnopharmacol, 2010, 129:167-173.
[9] Zhu H, Shen H, Xu J, et al. Comparative study on intestinal metabolism and absorption in vivo of ginsenosides in sulphurfumigated and non-fumigated ginseng by ultra performance liquid chromatography quadruple time-of-flight mass spectrometry based chemical profiling approach[J]. Drug Test Anal, 2015, 7:320-330.
[10] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (中华人民共和国药典)[S]. 2010 ed. Beijing:China Medical Science Press, 2010:160-161, 262-263.
[11] Chinese Pharmacopoeia Commission. Public notification for the limitation of sulfur fumigation residues in Chinese herbal medicines and pieces[J]. Drug Standards China (中国药品标准), 2011, 12:234.
[12] Wang G, Jin XJ, Wang H, et al. The influence of smoked and storage to Angelica sulfur dioxide and quality residues[J]. Lishizhen Med Mater Med Res (时珍国医国药), 2014, 25:453-455.
[13] Li XY, Xu JD, Xu J, et al. UPLC-QTOF-MS based metabolomics coupled with diagnostic ion exploration strategy for rapidly evaluating sulfur-fumigation caused holistic quality variation of medicinal herbs, Moutan Cortex as an exam[J]. Anal Methods, 2016, 8:1034-1043.
[14] Xu SJ, Yang L, Zeng X, et al. Characterization of compounds in the Chinese herbal drug Mu-Dan-Pi by liquid chromatography coupled to electrospray ionization mass spectrometry[J]. Rapid Commun Mass Spectrom, 2006, 20:3275-3288.
[15] Xu SJ, Yang L, Zeng M, et al. Rapid identification of compounds in Cortex Moutan by liquid chromatographytandem mass spectrometry[J]. Acta Pharm Sin (药学学报), 2006, 41:852-856.
[16] Xiao CN, Wu M, Chen YY, et al. Revealing metabolomic variations in Cortex Moutan from different root parts using HPLC-MS method[J]. Phytochem Anal, 2015, 26:86-93.
[17] Li SL, Song JZ, Choi FFK, et al. Chemical profiling of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight mass spectrometry[J]. J Pharm Biomed Anal, 2009, 49:253-266.
[18] Zhang J, Cai H, Cao G, et al. Exploring potential chemical transformation by chemical profiling approach for rapidly evaluating chemical consistency between sun-dried and sulfurfumigated Radix Paeoniae Alba using ultra performance liquid chromatography coupled with time-of-flight mass spectrometry[J]. Evid Based Complement Alternat Med, 2013, DOI:10.1155/2013/763213.
[19] Huang SJ, Wang R, Shi YH, et al. Primary safety evaluation of sulfated Paeoniae Radix Alba[J]. Acta Pharm Sin (药学学报), 2012, 47:486-491.
[20] Fu LQ, Wei XL. Comparative study of paeoniflorin and paeoniflorin sulfite metabolites[J]. Chin Hosp Pharm (中国医院药学杂志), 2014, 34:1656-1658.
[21] Wang Q, Liu RX, Guo HZ, et al. Study on influence of processing methods on chemical constituents in Radix Paeoniae Alba[J]. China J Chin Mater Med (中国中药杂志), 2006, 31:1418-1421.
[22] Bai YJ, Xu JD, Kong M, et al. Discovery of characteristic chemical markers for inspecting sulfur-fumigated Radix Angelicae Sinensis by ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry based metabolomics and chemical profiling approach.[J]. Food Res Int, 2015, 76:387-394.
[23] Li ZY, Gao HM, Sun J, et al. Secoiridoid sulfonates from the sulfiting-processed buds of Lonicera japonica[J]. Helv Chim Acta, 2012, 95:1144-1151.