药学学报, 2016, 51(9): 1458-1463
引用本文:
董巍, 郝修洁, 王超众, 郭丽娜, 潘虹, 林宇, 李晓明, 王喜军. 基于UHPLC-QTOF/MS和MetaboLynx分析的蜜炙对叶百部血清药物化学研究[J]. 药学学报, 2016, 51(9): 1458-1463.
DONG Wei, HAO Xiu-jie, WANG Chao-zhong, GUO Li-na, PAN Hong, LIN Yu, LI Xiao-ming, WANG Xi-jun. Serum pharmacochemistry of honey-fried Stemona tuberosa Lour. by UHPLC-QTOF/MS and MetaboLynx analysis[J]. Acta Pharmaceutica Sinica, 2016, 51(9): 1458-1463.

基于UHPLC-QTOF/MS和MetaboLynx分析的蜜炙对叶百部血清药物化学研究
董巍1, 郝修洁2, 王超众2, 郭丽娜1, 潘虹1, 林宇1, 李晓明1, 王喜军3
1. 齐齐哈尔医学院, 黑龙江 齐齐哈尔 161006;
2. 齐齐哈尔市食品药品检验检测中心, 黑龙江 齐齐哈尔 161006;
3. 黑龙江中医药大学, 黑龙江 哈尔滨 150040
摘要:
研究蜜炙对叶百部体内作用的物质基础,并建立蜜炙对叶百部体内外成分的UHPLC-QTOF/MS方法,借助MetaboLynx数据处理技术提取口服蜜炙对叶百部后大鼠血浆中血中移行成分微量信息,鉴定主要血中移行成分及其代谢产物。鉴定与表征了大鼠血中移行成分40个,包括12个原形成分和28个代谢产物,入血成分及代谢产物可能为蜜炙对叶百部体内直接作用的药效成分,蜜炙对叶百部血清药物化学的表征为阐明其蜜炙炮制的药理作用及其机制提供了依据。
关键词:    蜜炙对叶百部      血清药物化学      UHPLC-QTOF/MS     
Serum pharmacochemistry of honey-fried Stemona tuberosa Lour. by UHPLC-QTOF/MS and MetaboLynx analysis
DONG Wei1, HAO Xiu-jie2, WANG Chao-zhong2, GUO Li-na1, PAN Hong1, LIN Yu1, LI Xiao-ming1, WANG Xi-jun3
1. Qiqihar Medical University, Qiqihar 161006, China;
2. Qiqihar Institute for Food and Drug Control, Qiqihar 161006, China;
3. Heilongjiang University of Chinese Medicine, Harbin 150040, China
Abstract:
A high throughput UHPLC-QTOF/MS method was established to analyze and identify the constituents in rat plasma after oral administration of honey-fried Stemona tuberosa Lour. extract. Automated data analysis software MetaboLynx was developed in analysis and identification of the bioactive components and their metabolites in rat plasma following oral administration of honey-fried S. tuberosa Lour. extract. As a result, 40 compounds including 12 prototype components and 28 metabolites were characterized. The constituents absorbed into blood and the possible metabolites which were demonstrated to originate from the active fraction of honey-fried S. tuberosa Lour. were responsible for the observed efficacy. Its serum pharma­cochemistry should be subjected to complete investigation to illuminate the pharmacology and active mechanism of honey-fried S. tuberosa Lour.
Key words:    honey-fried Stemona tuberosa Lour.    serum pharmacochemistry    UHPLC-QTOF/MS   
收稿日期: 2016-03-16
DOI: 10.16438/j.0513-4870.2016-0231
基金项目: 黑龙江省教育厅科学技术研究项目(12541925)。
通讯作者: 王喜军
Email: xijunwangls@126.com
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