药学学报, 2016, 51(10): 1600-1608
引用本文:
苏联麟, 程雪, 季德, 汪丽君, 丁喜炎, 陆兔林, 毛春芹, 张季. 基于UHPLC-QTOF/MS技术的生、醋五味子醇提物在大鼠血浆、胆汁、尿液、粪便中药物原形成分及其代谢产物的分析鉴定[J]. 药学学报, 2016, 51(10): 1600-1608.
SU Lian-lin, CHENG Xue, JI De, WANG Li-jun, DING Xi-yan, LU Tu-lin, MAO Chun-qin, ZHANG Ji. Analysis of lignans and their metabolites derived from Schisandra chinensis and vinegar Schisandra chinensis in rats' plasma, bile, urine and faeces based on UHPLC-QTOF/MS[J]. Acta Pharmaceutica Sinica, 2016, 51(10): 1600-1608.

基于UHPLC-QTOF/MS技术的生、醋五味子醇提物在大鼠血浆、胆汁、尿液、粪便中药物原形成分及其代谢产物的分析鉴定
苏联麟1, 程雪1, 季德1,2, 汪丽君1, 丁喜炎1, 陆兔林1,2, 毛春芹1, 张季1
1. 南京中医药大学药学院, 江苏 南京 210023;
2. 南京中医药大学江苏省中药炮制重点实验室, 江苏 南京 210023
摘要:
采用UHPLC-QTOF/MS技术研究五味子生品及炮制品(醋制)的醇提取物在大鼠体内药物原形成分及其代谢产物的差异性,利用质量亏损滤过(MDF)策略以及动态背景消除(DBS)、加强峰提取(EPL)等数据处理技术进行分析,鉴定大鼠灌胃给予生、醋五味子提取物后血浆、尿液、胆汁及粪便中原形成分及其代谢产物。为了更好地与五味子保肝效应联系起来,本实验建立了大鼠急性酒精性肝损伤模型,首次进行病理状态下动物体内五味子木脂素的代谢研究。五味子醇提物中主要成分是木脂素,包括五味子甲素、五味子乙素、五味子丙素、五味子醇甲、五味子醇乙、五味子酯甲、五味子酯乙、五味子酚、戈米辛G、戈米辛J等。木脂素在大鼠体内主要发生去甲基化、羟基化、氧化等代谢转化,最终在生、醋五味子组中共鉴定出6种木脂素原形成分及它们的20种代谢产物。
关键词:    五味子      醋制      UHPLC-QTOF/MS      木脂素      代谢产物     
Analysis of lignans and their metabolites derived from Schisandra chinensis and vinegar Schisandra chinensis in rats' plasma, bile, urine and faeces based on UHPLC-QTOF/MS
SU Lian-lin1, CHENG Xue1, JI De1,2, WANG Li-jun1, DING Xi-yan1, LU Tu-lin1,2, MAO Chun-qin1, ZHANG Ji1
1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;
2. Jiangsu Provincial Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China
Abstract:
UHPLC-QTOF/MS technique was used to study the differences of lignans and their metabolites derived from Schisandra chinensis and vinegar Schisandra chinensis in rat plasma, bile, urine and faeces by the data processing techniques such as the dynamic background subtract (DBS), mass defect filtering (MDF) and enhance peak list (EPL) in analysis. In order to enhance accuracy for Schisandra chinensis hepatoprotective effect, we established rat acute alcoholic liver injury model in this experiment, and studied the prototype components and metabolisms of Schisandra lignans in vivo under pathological condition. The main ingredients of alcohol extract are lignans, including deoxyschizandrin, schisandrin B, schizandrin C, schizandrol, schizandrol B, schisantherin, schisantherin B, schisanhenol, gomisin G, gomisin J. The metabolic transformation of lignans in rats was mainly induced by methylation, hydroxyl, oxidation, and so on. Finally, we identified 6 kinds of prototype components and their 20 potential metabolites in Schisandra chinensis group and vinegar Schisandra chinensis group.
Key words:    Schisandra chinensis    processing with rice vinegar    UHPLC-QTOF/MS    lignans    metabolite   
收稿日期: 2016-04-21
DOI: 10.16438/j.0513-4870.2016-0315
基金项目: 国家“重大新药创制”科技重大专项资助项目(2012ZX09304005-002);国家自然科学基金资助项目(81373971);江苏省中医药管理局科技项目(LZ13022);江苏省高校优势学科项目(2011ZYX2-003);南京中医药大学研究生创新工程项目(CXLX13_597).
通讯作者: 陆兔林
Email: lutuling2005@126.com
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