药学学报, 2016, 51(10): 1609-1615
引用本文:
常相伟, 李德坤, 王彤, 吴亚超, 赵颖, 周大铮, 张涛, 叶正良. 基于UHPLC-QTOF/MS的植物代谢组学技术鉴别林下山参的不同部位[J]. 药学学报, 2016, 51(10): 1609-1615.
CHANG Xiang-wei, LI De-kun, WANG Tong, WU Ya-chao, ZHAO Ying, ZHOU Da-zheng, ZHANG Tao, YE Zheng-liang. Application of metabolomics approach to study of different parts of Mountain Cultivated Ginseng using UHPLC-QTOF/MS[J]. Acta Pharmaceutica Sinica, 2016, 51(10): 1609-1615.

基于UHPLC-QTOF/MS的植物代谢组学技术鉴别林下山参的不同部位
常相伟1, 李德坤2, 王彤3, 吴亚超1, 赵颖2, 周大铮2, 张涛4, 叶正良2
1. 天津中医药大学, 天津 300193;
2. 天津天士力之骄药业有限公司, 天津 300410;
3. 天津医科大学, 天津 300070;
4. 辽宁天士力森涛参茸股份有限公司, 辽宁 本溪 117206
摘要:
为了对林下山参的不同部位进行鉴别,本研究采用基于UHPLC-QTOF/MS的植物代谢组学技术,并结合PCA和OPLS-DA分析方法对林下山参叶、主根、侧根、芦头等4个部位的代谢物进行研究。PCA得分图显示林下山参叶、主根、侧根和芦头被明显分为4类,表明林下山参不同部位之间化学成分差异显著。从林下山参4个不同部位中共检测到81个主要代谢物,其中包括林下山参叶与根茎(主根、侧根、芦头)之间的差异标志物9个,林下山参主根与侧根、主根与芦头之间的差异标志物各7个,通过将这些代谢物的保留时间、精确分子量及碎片离子与对照品和相关人参属文献报道的化合物进行匹配,最终鉴定或初步推断出70个代谢物。本研究建立了一种快速、准确、可靠的林下山参不同部位的鉴别方法,同时也为其他中药材质量评价提供了一种新的思路。
关键词:    林下山参      UHPLC-QTOF/MS      植物代谢组学      正交偏最小二乘判别分析      差异标志物     
Application of metabolomics approach to study of different parts of Mountain Cultivated Ginseng using UHPLC-QTOF/MS
CHANG Xiang-wei1, LI De-kun2, WANG Tong3, WU Ya-chao1, ZHAO Ying2, ZHOU Da-zheng2, ZHANG Tao4, YE Zheng-liang2
1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
2. Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300410, China;
3. Tianjin Medical University, Tianjin 300070, China;
4. Liaoning Tasly Sentaoshenrong Co., Ltd., Benxi 117206, China
Abstract:
In present study, an ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS)-based plant metabolomics approach was established to investigate the metabolic profiles of the leaves, main root, branch root, and rhizome of Mountain Cultivated Ginseng (MCG). The UHPLC-QTOF/MS data were subjected to principal component analysis (PCA) and orthogonal partial least squared discrimination analysis (OPLS-DA) to find the potential characteristic components of the four parts of MCG in a quick way. The four different parts could be separated into four different groups of phytochemicals according to the PCA scores. The chemical constituents in four parts of MCG were obviously different. The identities of 81 major peaks that were detected in the four parts of MCG and the potential markers were identified by comparison with the reference compounds or were tentatively assigned by matching the retention time,empirical molecular formula and fragment ions with those of the published compounds of the Panax species. This proposed analytical method is fast, accurate, and reliable for differentiating the different parts of MCG. Moreover, this study supplied a new method for the quality evaluation of other Chinese medicinal materials.
Key words:    Mountain Cultivated Ginseng    UHPLC-QTOF/MS    plant metabolomics    OPLS-DA    potential marker   
收稿日期: 2016-04-08
DOI: 10.16438/j.0513-4870.2016-0325
基金项目: “重大新药创制”国家科技重大专项资助项目(2013ZX09402202).
通讯作者: 叶正良
Email: yezl@tasly.com
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