药学学报, 2017, 52(1): 126-131
引用本文:
樊佳新, 王帅, 孟宪生, 包永睿, 李天娇. 基于微流控芯片技术的荆芥诱导肺肿瘤细胞凋亡谱效关系研究[J]. 药学学报, 2017, 52(1): 126-131.
FAN Jia-xin, WANG Shuai, MENG Xian-sheng, BAO Yong-rui, LI Tian-jiao. Study of cancer cell apoptosis induced by Schizonepeta tenuifolia with microfluidic chip technology[J]. Acta Pharmaceutica Sinica, 2017, 52(1): 126-131.

基于微流控芯片技术的荆芥诱导肺肿瘤细胞凋亡谱效关系研究
樊佳新1, 王帅1,2,3, 孟宪生1,2,3, 包永睿1,2,3, 李天娇1,2,3
1. 辽宁中医药大学药学院, 辽宁 大连 116600;
2. 辽宁中医药大学 辽宁省组分中药工程技术研究中心, 辽宁 大连 116600;
3. 辽宁中医药大学 辽宁省现代中药研究工程实验室, 辽宁 大连 116600
摘要:
为了进一步全面地阐明荆芥药材乙醇提取物中主要发挥抗肺癌作用的化学组成,本文基于微流控芯片技术开展不同产地荆芥药材乙醇提取物的体外抗肺肿瘤药效学研究,并通过HPLC方法建立9种不同产地荆芥药材乙醇提取物的指纹图谱,将药效指标与指纹图谱的相关信息通过灰色关联度软件进行谱效关系分析,采用HPLC-Q-TOF/MS的技术手段对其进行快速分析与鉴定。结果显示,关联系数较大的色谱峰中第19、6、16、11、18号峰所代表的化合物为香叶木素、木犀草苷、木犀草素、橙皮苷、芹菜素;第10、12、20号峰可能为柚皮素-7-O-葡萄糖醛酸苷或槲皮苷、迷迭香酸甲酯或异鼠李素-3-木糖苷、5,7-二羟基-6,4-二甲氧基黄酮。该研究进一步明确了荆芥中发挥抗肺肿瘤作用的主要化学组成,为荆芥药材的质量控制及中药复杂成分的药效物质基础研究提供方法学探讨和实验依据。
关键词:    荆芥      微流控芯片      A549细胞      谱效关系      灰色关联度     
Study of cancer cell apoptosis induced by Schizonepeta tenuifolia with microfluidic chip technology
FAN Jia-xin1, WANG Shuai1,2,3, MENG Xian-sheng1,2,3, BAO Yong-rui1,2,3, LI Tian-jiao1,2,3
1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;
2. Liaoning Province Multi-Component Chinese Medicine Engineering Technology Research Center, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;
3. Liaoning Province Modern Traditional Chinese Medicine Research and Engineering Laboratory, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
Abstract:
This study was designed to elucidate the chemical composition and anti-cancer effects of Schizonepeta tenuifolia's ethanol extracts. Microfluidic technology was used in the study of Schizonepeta tenuifolia from 9 different geographic regions. The ethanol extracts were examined with HPLC to establish their Fingerprints in order to analyze the relationship between the spectrum and efficacy index through Grey Correlation software, and a rapid HPLC-Q-TOF/MS method was established. The result shows that chromatographic peaks of the 19, 6, 11, 16, 18th are the representative diosmetin, luteoloside, hesperidin, luteolin, and apigenin. The 10, 12, 20th peaks may be naringenin-7-O-glucuronide or quercitrin, rosmarinate or acetylcorynoline, and 5,7-dihydroxy-6,4-dimethoxy flavone. The major chemical composition of Schizonepeta tenuifolia was found to have the anti-lung-tumor effects. A new method was established for the quality control of traditional Chinese medicine.
Key words:    Schizonepeta tenuifolia    microfluidic chip    A549 cell    spectrum effect relationship    grey correlation degree   
收稿日期: 2016-08-24
DOI: 10.16438/j.0513-4870.2016-0466
基金项目: “十二五”“重大新药创制”科技重大专项资助项目(2013ZX09507005);辽宁省一般项目资助项目(L2014361,L201605).
通讯作者: 孟宪生,Tel/Fax:86-411-85890185,E-mail:mxsvvv@126.com
Email: mxsvvv@126.com
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