药学学报, 2017, 52(1): 132-138
引用本文:
韩彦琪, 许浚, 龚苏晓, 张铁军, 刘昌孝. HPLC-QTOF/MS方法分析元胡止痛方的化学成分[J]. 药学学报, 2017, 52(1): 132-138.
HAN Yan-qi, XU Jun, GONG Su-xiao, ZHANG Tie-jun, LIU Chang-xiao. Identification of chemical constituents of Yuanhu Zhitong prescription by HPLC-QTOF/MS[J]. Acta Pharmaceutica Sinica, 2017, 52(1): 132-138.

HPLC-QTOF/MS方法分析元胡止痛方的化学成分
韩彦琪1, 许浚1, 龚苏晓1, 张铁军1, 刘昌孝2
1. 天津药物研究院 天津市中药质量控制技术工程实验室, 天津 300193;
2. 天津药物研究院新药评价中心, 释药技术与药代动力学国家重点实验室, 天津 300193
摘要:
为全面阐明元胡止痛方的化学成分,本研究利用高效液相色谱串联四级杆/飞行时间质谱仪(HPLC-QTOF/MS)对元胡止痛方的化学组成进行分析鉴定。通过QTOF/MS提供化合物的准确分子质量、碎片离子信息,结合参考文献及部分对照品保留时间和质谱数据,共鉴定出元胡止痛方中51个化合物,包括28个生物碱类成分和23个香豆素类成分。本研究快速、准确、系统地分析了元胡止痛方的化学组分,为其质量控制和药效物质研究奠定基础。
关键词:    元胡止痛方      HPLC-QTOF/MS      化学成分      定性分析     
Identification of chemical constituents of Yuanhu Zhitong prescription by HPLC-QTOF/MS
HAN Yan-qi1, XU Jun1, GONG Su-xiao1, ZHANG Tie-jun1, LIU Chang-xiao2
1. Tianjin Engineering Laboratory of Quality Control Technology of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research, Tianjin, 300193, China;
2. Tianjin Center for New Drug Evaluation and Research, State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin, 300193, China
Abstract:
This study was designed to clarify the chemical constituents in Yuanhu Zhitong prescription (YHZT), a rapid high performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (HPLC-QTOF/MS) method was established. Based on the high resolution MS spectra data, fragmentation ion information, reference standards data and literature reports, 51 peaks including 28 alkaloid compounds and 23 coumarin compounds were identified. The chemical constituents in YHZT were rapidly, accurately, systematically analyzed. The results lay a foundation for the quality control of effective compounds of YHZT.
Key words:    Yuanhu Zhitong prescription    HPLC-QTOF/MS    chemical constituent    qualitative analysis   
收稿日期: 2016-06-30
DOI: 10.16438/j.0513-4870.2016-0594
基金项目: 国家自然科学基金重点项目(81430096);天津市自然科学基金资助项目(16JCYBJC28300).
通讯作者: 张铁军,Tel:86-22-23006848,E-mail:zhangtj@tjipr.com;刘昌孝,E-mail:liuchangxiao@163.com
Email: zhangtj@tjipr.com;liuchangxiao@163.com
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