药学学报, 2018, 53(3): 396-402
引用本文:
柴煊, 孟雅坤, 柏兆方, 张雅铭, 肖小河, 牛明. 基于生物靶标网络分析的山豆根抗乙肝病毒的作用机制初步研究[J]. 药学学报, 2018, 53(3): 396-402.
CHAI Xuan, MENG Ya-kun, BAI Zhao-fang, ZHANG Ya-ming, XIAO Xiao-he, NIU Ming. Mechanism of anti-hepatitis B virus activity of Tonkinensis based on biological targets network[J]. Acta Pharmaceutica Sinica, 2018, 53(3): 396-402.

基于生物靶标网络分析的山豆根抗乙肝病毒的作用机制初步研究
柴煊1,2,3, 孟雅坤2, 柏兆方2, 张雅铭2, 肖小河2, 牛明2
1. 解放军军事医学科学院, 北京 100850;
2. 解放军302医院全军中医药研究所, 北京 100039;
3. 武警北京总队第三医院, 北京 100141
摘要:
山豆根是一味常用于治疗乙肝的中药,在改善症状、保肝降酶、利胆退黄等方面疗效确切,但其抗乙肝病毒的作用机制尚不明确。为此,本文基于系统生物学的思想,通过整合网络药理学与代谢组学两种技术,较为系统地分析山豆根抗病毒的主要作用机制。首先,通过预测山豆根中主要化学成分的作用靶标,建立山豆根的“药物成分-靶标”网络;其次,以人肝癌细胞HepG2.2.15为研究对象,通过代谢组学技术分析山豆根抗乙肝的主要代谢过程和其主要影响的代谢物;最后,通过构建“药物成分-靶标-代谢物”网络,分析山豆根抗乙肝的主要作用靶标及代谢物,进而阐明其主要作用机制。山豆根可能主要作用于16个靶标蛋白发挥抗乙肝病毒作用,其作用机制可能与调节视黄醇代谢、过氧化物酶体增殖物激活型受体(peroxisome proliferator activate-receptors,PPAR)信号通路、癌症中的转录失调等过程有关,从而达到控制乙肝病毒复制、调节机体免疫和代谢功能紊乱等作用。
关键词:    山豆根      网络药理学      代谢组学      乙肝病毒     
Mechanism of anti-hepatitis B virus activity of Tonkinensis based on biological targets network
CHAI Xuan1,2,3, MENG Ya-kun2, BAI Zhao-fang2, ZHANG Ya-ming2, XIAO Xiao-he2, NIU Ming2
1. Academy of Military Medical Sciences, Beijing 100850, China;
2. China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing 100039, China;
3. The 3th Hospital of Chinese Armed Police Force in Beijing, Be ijing 100141, China
Abstract:
Tonkinensis is commonly used in the treatment of hepatitis B infection in China with its effect­tiveness in reducing clinical symptoms and improving liver function. However, the mechanism of the anti-HBV (hepatitis B virus) effect of Tonkinensis is still not clear. In this study, an integrative analysis using the network pharmacology and metabolomics was employed in identification of the main targets and mechanisms of Tonkinensis in treatment of HBV infections. First, the "drug-target" network was established by predicting the targets of the main chemical components of Tonkinensis; Secondly, the differential metabolites associated with the anti-HBV effect of Tonkinensis were analyzed with the LC-MS based metabolomics in HepG2.2.15 cells; Finally, the "drug ingredients-targets-metabolites" network was constructed to screen the main anti-HBV targets of Tonkinensis. The results suggest that Tonkinensis may act on 16 target proteins in the network of retinol metabolism, peroxisome proliferator activate-receptors (PPAR) signaling pathway and transcriptional regulation of cancer and so on, which contributed to the control of HBV replication and the regulation of immune function and metabolic disorders.
Key words:    Tonkinensis    network pharmacology    metabolomics    hepatitis B virus   
收稿日期: 2017-11-14
DOI: 10.16438/j.0513-4870.2017-1124
基金项目: 国家自然科学基金资助项目(81330090,81503247,81721002);中医药行业科研专项(201507002);全军医学科技“十二五”科研项目(BWS11J049).
通讯作者: 肖小河,Tel:86-10-66933325,E-mail:nmbright@163.com;牛明,Tel:86-10-66933322,E-mail:pharmacy302@126.com
Email: nmbright@163.com;pharmacy302@126.com
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