药学学报, 2018, 53(4): 538-545
周婉祎, 赵一秀, 安娜, 白云龙, 张妍, 杨宝峰. 花青素对晶状体上皮细胞氧化应激损伤和上皮间充质转化的抑制作用[J]. 药学学报, 2018, 53(4): 538-545.
ZHOU Wan-yi, ZHAO Yi-xiu, AN Na, BAI Yun-long, ZHANG Yan, YANG Bao-feng. Inhibitory effects of anthocyanin on oxidative stress injury and epithelial-mesenchymal transition of human lens epithelial cells[J]. Acta Pharmaceutica Sinica, 2018, 53(4): 538-545.

周婉祎1, 赵一秀1, 安娜2, 白云龙1, 张妍1, 杨宝峰1
1. 哈尔滨医科大学药学院, 省部共建国家重点实验室培育基地, 心血管药物研究教育部重点实验室, 黑龙江 哈尔滨 150081;
2. 哈尔滨医科大学黑龙江省医学科学院, 黑龙江 哈尔滨 150081
花青素(anthocyanin)是广泛存在于植物中的一种水溶性黄酮类色素。研究表明花青素对视力具有一定的保护作用,但其对于白内障是否具有治疗作用及机制尚不明确。本研究分别采用过氧化氢(hydrogen peroxide,H2O2)及转化生长因子-β2(transforming growth factor β2,TGF-β2)处理人晶状体上皮细胞(human lens epithelial cells,HLECs),诱导氧化应激损伤及上皮-间充质转化(epithelial-mesenchymal transition,EMT),以构建年龄相关性白内障(age-related cataract,ARC)及后囊膜浑浊型白内障(posterior capsule opacification,PCO)细胞模型,并检测花青素对HLECs氧化应激损伤及EMT标志物的影响。结果表明,花青素有较好的抗氧化应激损伤作用,能明显保护细胞活力,增加细胞内SOD、GSSG、GSH-Px及α-晶状体蛋白水平,降低细胞内MDA、ROS及Ca2+水平;同时,花青素显著抑制后囊膜浑浊型白内障发生发展的病理进程,能明显抑制TGF-β2诱导的HLECs增殖、迁移,降低EMT标志物COL1A1、COL1A2、COL3、COL4、Fn、α-SMA的mRNA表达水平。综上,花青素能保护HLECs细胞对抗H2O2诱导的自由基损伤及由TGF-β2诱导的EMT,对ARC及PCO具有一定的预防和治疗作用。
关键词:    花青素      白内障      氧化应激      上皮间充质转化      细胞增殖     
Inhibitory effects of anthocyanin on oxidative stress injury and epithelial-mesenchymal transition of human lens epithelial cells
ZHOU Wan-yi1, ZHAO Yi-xiu1, AN Na2, BAI Yun-long1, ZHANG Yan1, YANG Bao-feng1
1. State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin 150081, China;
2. Heilongjiang Medical Academy, Harbin Medical University, Harbin 150081, China
Anthocyanin is a water-soluble flavonoid pigment which is widely found in plants. Studies showed that anthocyanin had protective effect on vision. However, whether anthocyanin has therapeutic effect on cataract remain unclear. In this study, we established the age-related and posterior capsule opacification cataract cell models through inducing oxidative damage of human lens epithelial cells (HLECs) by H2O2 and inducing epithelial-mesenchymal transition (EMT) by transforming growth factor β2 (TGF-β2). The preventative effects of anthocyanin on markers of oxidative damage and EMT were determined by respective assay kits and PCR analysis. Anthocyanin was beneficial to reduce oxidative stress of HLECs, protecting cells from H2O2 induced damage and increasing α-crystallin expression. The potential mechanisms might be that anthocyanin increased the activities of SOD and GSH-Px, which contributes to reduce cellular ROS and MDA level. Besides, anthocyanin inhibited Ca2+ overload, which contributes to protection of cell from apoptosis. Meanwhile, anthocyanin had inhibitory effect on EMT, slowed down cell proliferation, migration caused by TGF-β2 through decreasing mRNA expression levels of EMT markers including COL1A1, COL1A2, COL3, COL4, Fn and α-SMA. The results suggest that anthocyanin could protect HLECs from oxidative damage induced by H2O2 and cell proliferation, migration and EMT induced by TGF-β2, which indicated that anthocyanin may have protective and therapeutic effects on age-related cataract and posterior capsule opacification.
Key words:    anthocyanin    cataract    oxidative stress    epithelial-mesenchymal transition    cell proliferation   
收稿日期: 2017-10-15
DOI: 10.16438/j.0513-4870.2017-1004
基金项目: 中央引导地方科技发展专项资助项目(ZY16A07).
通讯作者: 张妍,Tel:86-451-86671354,E-mail:zhangyan@ems.hrbmu.edu.cn
Email: zhangyan@ems.hrbmu.edu.cn
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