药学学报, 2018, 53(4): 598-603
引用本文:
黄莉莉, 昝珂, 过立农, 刘杰, 郑健, 马双成. 基于核苷类成分对不同商品等级冬虫夏草的比较研究[J]. 药学学报, 2018, 53(4): 598-603.
HUANG Li-li, ZAN Ke, GUO Li-nong, LIU Jie, ZHENG Jian, MA Shuang-cheng. Comparative study on nucleosides in circulated Cordyceps sinensis of different sizes[J]. Acta Pharmaceutica Sinica, 2018, 53(4): 598-603.

基于核苷类成分对不同商品等级冬虫夏草的比较研究
黄莉莉2, 昝珂1, 过立农1, 刘杰1, 郑健1, 马双成1,2
1. 中国食品药品检定研究院, 北京 100050;
2. 广西壮族自治区食品药品检验所, 广西 南宁 530021
摘要:
建立不同商品等级冬虫夏草指纹图谱及含量测定的方法,对5个产地的冬虫夏草进行比较研究,分析其相似度及其主要核苷(尿苷、肌苷、鸟苷和腺苷)的含量,以梳理不同等级冬虫夏草的关系。采用Waters XSelect HSS T3 C18色谱柱(4.6 mm×250 mm,5 μm);水(A)-乙腈(B)为流动相,梯度洗脱(0~5 min,0 B;5~15 min,0→10% B,15~30 min,10%→20% B;30~33 min,20%→50% B;33~35 min,50%→0 B;35~40 min,0 B),流速0.6 mL·min-1,检测波长为260 nm,柱温30℃,进样量5 μL。结果表明,相同产地不同商品等级冬虫夏草中核苷类成分无显著性差异,但不同产地冬虫夏草中核苷类成分的相似度比较及含量存在较大差异,有待收集更多数据进一步研究。本方法为不同商品等级冬虫夏草的使用提供了科学依据,并为其商品级别质量标准的制订提供参考。
关键词:    冬虫夏草      不同商品等级      指纹图谱      核苷     
Comparative study on nucleosides in circulated Cordyceps sinensis of different sizes
HUANG Li-li2, ZAN Ke1, GUO Li-nong1, LIU Jie1, ZHENG Jian1, MA Shuang-cheng1,2
1. National Institutes for Food and Drug Control, Beijing 100050, China;
2. Guangxi Institute for Food and Drug Control, Nanning 530021, China
Abstract:
This study was designed to establish a method to obtain the fingerprint chromatogram for the quantitative determination of Cordyceps sinensis in different sizes, a comparison of Cordyceps sinensis from five places was made to analyze its similarity and the content of main nucleosides (uridine, inoside, guanosine and adenosine). The assay was performed on a Waters XSelect HSS T3 C18 (4.6 mm×250 mm, 5 μm), with a mobile phase consisting of water (A)-acetonitrile (B) at the flow rate of 0.6 mL·min-1 (0-5 min, 0 B; 5-15 min, 0→10% B; 15-30 min, 10%→20% B; 30-33 min, 20%→50% B; 33-35 min, 50%→0 B; 35-40 min, 0 B). The detection wavelength was 260 nm and the column temperature was set at 30℃, and the injection volume was 5 μL. The results showed that there was no significant difference of the nucleosides in samples from the same place of the different sizes, but contents of the nucleosides variate a lot by production places. More data are required for further research. The method is proved for scientific and specific formulation of the standard in evaluation of circulated Cordyceps sinensis.
Key words:    Cordyceps sinensis    different sizes    fingerprint chromatogram    nucleosides   
收稿日期: 2017-10-21
DOI: 10.16438/j.0513-4870.2017-0940
基金项目: 中医药行业科研专项"中药饮片质量保障系统研究(一)"资助(201507002);十二五国家科技重大专项(2014ZX09304307-002).
通讯作者: 郑健,Tel:86-10-67095739,E-mail:bjzj825@163.com;马双成,Tel:86-10-67095272,E-mail:masc@nifdc.org.cn
Email: bjzj825@163.com;masc@nifdc.org.cn
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