药学学报, 2018, 53(4): 630-635
引用本文:
崔祥, 尚尔鑫, 江曙, 钱大玮, 段金廒. 基于响应曲面法对黄芩-黄连配伍改善2型糖尿病糖脂代谢紊乱的相互作用研究[J]. 药学学报, 2018, 53(4): 630-635.
CUI Xiang, SHANG Er-xin, JIANG Shu, QIAN Da-wei, DUAN Jin-ao. Interaction of Scutellaria-Coptis herb pair in improvement of glucose and lipid metabolism of type 2 diabetes rats based on response surface method[J]. Acta Pharmaceutica Sinica, 2018, 53(4): 630-635.

基于响应曲面法对黄芩-黄连配伍改善2型糖尿病糖脂代谢紊乱的相互作用研究
崔祥, 尚尔鑫, 江曙, 钱大玮, 段金廒
南京中医药大学, 江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023
摘要:
应用响应曲面法阐明黄芩-黄连药对改善糖脂代谢的相互作用范围、性质与程度。采用高糖高脂饲料喂养,联合注射低剂量链脲佐菌素(STZ),建立2型糖尿病大鼠模型,给予不同配比(1:0、3:1、2:1、3:2、1:1、2:3、1:2、1:3、0:1)、不同剂量(低、高)黄芩-黄连药对提取液后,观察其对2型糖尿病大鼠糖脂代谢各指标的影响,采用多指标综合指数法对各指标进行整合,应用响应曲面法对整合结果进行分析。结果表明,黄芩-黄连在3:1~1:3比例范围内均有一定的协同作用(相互作用值:-0.2~-0.8),尤其在二者比例为3:2~1:3时,具有显著的协同作用(相互作用值:-0.8),且1:1高剂量组的协同效应较强。
关键词:    黄芩      黄连      药对      糖脂代谢      响应曲面法     
Interaction of Scutellaria-Coptis herb pair in improvement of glucose and lipid metabolism of type 2 diabetes rats based on response surface method
CUI Xiang, SHANG Er-xin, JIANG Shu, QIAN Da-wei, DUAN Jin-ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources, Nanjing University of Chinese Medicine, Nanjing 210023, China
Abstract:
The response surface method was used to evaluate the concentration range, property and degree of Scutellaria (HQ) and Coptis (HL) herb pair on improvement of glucose and lipid metabolism in this paper. Type 2 diabetes mellitus (T2DM) was induced in rats by low a dose of streptozotocin (STZ) in combination with high-fat diet feeding. The rats were administered with HQ-HL extract pairs at different ratio (1:0, 3:1, 2:1, 3:2, 1:1, 2:3, 1:2, 1:3, 0:1). To investigate the influence of herb pair extracts on glucose and lipid metabolism of T2DM rats, the multi index synthetic index method was adopted to integrate the indexes, then the response surface method was applied to analyze the integration results. The ratio of HQ and HL from 3:1 to 1:3 showed a synergistic effect (with the value between -0.2 and -0.8). Especially, when the proportion was between 3:2 and 1:3, herb pair extracts possessed stronger synergistic effects (value:-0.8). The high dose of HQ-HL with the ratio of 1:1 exhibited the strongest synergistic effect.
Key words:    Scutellaria    Coptis    herb pair    glucose and lipid metabolism    response surface method   
收稿日期: 2017-09-20
DOI: 10.16438/j.0513-4870.2017-0935
基金项目: 国家自然科学基金资助项目(81673831).
通讯作者: 江曙,Tel:86-25-85811516,E-mail:jiangshu2020@126.com
Email: jiangshu2020@126.com
相关功能
PDF(243KB) Free
打印本文
0
作者相关文章
崔祥  在本刊中的所有文章
尚尔鑫  在本刊中的所有文章
江曙  在本刊中的所有文章
钱大玮  在本刊中的所有文章
段金廒  在本刊中的所有文章

参考文献:
[1] Wang XJ. Methodology for systematic analysis of in vivo efficacy material base of traditional Chinese medicine Chinmedomics[J]. Chin J Chin Mater Med (中国中药杂志), 2015, 40:13-17.
[2] Wang J, Guo LL, Yang G, et al. Methodology and prospects of study on theory of compatibility of prescriptions in traditional Chinese medicine[J]. World Sci Technol (世界科学与技术), 2006, 8:1-5.
[3] Li WX, Tang YP, Shi XQ, et al. Research on Chinese medicine pairs (IV)-their compatibility effects[J]. Chin J Chin Mater Med (中国中药杂志), 2013, 38:4203-4207.
[4] Zhang XL, Zhou MM, Jia W, et al. Survey of modern research on Coptis root Scutellaria root and their drugs in a prescription[J]. Jilin J Tradit Chin Med (吉林中医药), 2010, 30:163-166.
[5] Pirillo A, Catapano AL. Berberine, a plant alkaloid with lipid-and glucose-lowering properties:from in vitro evidence to clinical studies[J]. Atherosclerosis, 2015, 243:449-461.
[6] Zhao L, Cang Z, Sun H, et al. Berberine improves gluco-genesis and lipid metabolism in nonalcoholic fatty liver dis-ease[J]. BMC Endocr Disord, 2017, 17:13.
[7] Zhang Z, Zhang H, Li B, et al. Berberine activates thermogenesis in white and brown adipose tissue[J]. Nat Commun, 2014, 5:5493.
[8] Song KH, Lee SH, Kim BY, et al. Extracts of Scutellaria baicalensis reduced body weight and blood triglyceride in db/db mice[J]. Phytother Res, 2013, 27:244-250.
[9] Zhang CH, Xu GL, Liu YH, et al. Anti-diabetic activities of Gegen Qinlian Decoction in high-fat diet combined with streptozotocin-induced diabetic rats and in 3T3-L1 adipocytes[J]. Phytomedicine, 2013, 20:221-229.
[10] Quan L, Lu YJ, Wan QP, et al. Study on evaluation index system and weight of management effect of diabetes mellitus[J]. Chin J Health Stati (中国卫生统计), 2014, 31:663-666.
[11] American Diabetes Association. Diagnosis and classification of diabetes mellitus[J]. Diabetes Care, 2014, 37:S81-S90.
[12] Zhu C, Zhu YY. Animal models of type Ⅱ diabetes:an overview[J]. Acta Lab Anim Sci Sin (中国实验动物学报), 2013, 21:84-88.
[13] Yu T, Sungelo MJ, Goldberg IJ, et al. Streptozoto-cin-treated high fat fed mice:a new type 2 diabetes model used to study canagliflozin-induced alterations in lipids and lipoproteins[J]. Horm Metab Res, 2017, 49:400-406.
[14] Huang MY, Tang YP, Shang EX, et al. Study on interaction of activating blood effects of the herb pair constituted by danggui and chuanxiong by response surface method[J]. Chin Pharmacol Bull (中国药理学通报), 2012, 28:1407-1413.
[15] Jin Y, Pu ZJ, Tang YP, et al. Effect of promoting blood circulation of herb pair containing Angelicae Sinensis Radix and Carthami Flos[J]. Chin Tradit Herb Drugs (中草药), 2017, 48:2087-2092.
相关文献:
1.罗奇志 罗佳波 王有志.高效液相色谱-电喷雾串联四极杆质谱定性分析双黄连粉针中化学成分及其药味归属[J]. 药学学报, 2009,44(12): 1391-1396
2.李伟;宋凤瑞;刘志强;刘淑莹.毛细管电泳及液相色谱法研究黄连黄芩配伍过程化学成分的变化[J]. 药学学报, 2008,43(2): 191-194
3.邓雅婷;廖琼峰;毕开顺;姚美村;姜晓飞;谢智勇.黄连-吴茱萸药对化学成分的HPLC-DAD-MS分析[J]. 药学学报, 2008,43(3): 299-302
4.王新宏;安睿;邹云;张艺;王智华;洪筱坤.双黄连片的多组分质量分析[J]. 药学学报, 2001,36(12): 917-920
5.程智勇;韩凤梅;蔡敏;陈勇.HPCE法测定双黄连口服液中的黄芩苷和绿原酸[J]. 药学学报, 1999,34(11): 854-857