药学学报, 2018, 53(6): 1009-1015
引用本文:
魏似婕, 赵志礼, 倪梁红, 吴靳荣, 高娜娜, 嘎务. 藏药“巴莴色保”基原鉴定及其叶绿体全基因组序列分析[J]. 药学学报, 2018, 53(6): 1009-1015.
WEI Si-jie, ZHAO Zhi-li, NI Liang-hong, WU Jin-rong, GAO Na-na, GAAWE Dorje. Taxonomic identification of Tibetan herb Bawo Sebo and its chloroplast genome structure[J]. Acta Pharmaceutica Sinica, 2018, 53(6): 1009-1015.

藏药“巴莴色保”基原鉴定及其叶绿体全基因组序列分析
魏似婕1, 赵志礼1, 倪梁红1, 吴靳荣1, 高娜娜1, 嘎务2
1. 上海中医药大学中药学院, 上海 201203;
2. 西藏藏医学院, 西藏 拉萨 850000
摘要:
常用藏药之一“巴莴色保”,根入药,治疗“黄水病”等。在民族植物学考察基础上,首次确定其基原植物的系统学位置——龙胆科獐牙菜属轮叶獐牙菜Swertia verticillifolia T.N.Ho et S.W.Liu;物种鉴定要点:基生叶发达,茎生叶轮生;花冠钟状,黄绿色,4裂,每裂片具裸露腺窝1。作为我国高山特有物种,首次得到其叶绿体全基因组序列:全长为151 682 bp,大单拷贝区(LSC)、小单拷贝区(SSC)分别为82 623 bp和18 335 bp,反向互补重复区(IR)为25 362 bp。注释叶绿体基因129个,其中84个编码蛋白基因、37个tRNA基因、8个rRNA基因。该工作可为“巴莴色保”品种鉴定、活性物质基础研究、质量标准制订及原植物就地保护等工作提供基础资料。
关键词:   
Taxonomic identification of Tibetan herb Bawo Sebo and its chloroplast genome structure
WEI Si-jie1, ZHAO Zhi-li1, NI Liang-hong1, WU Jin-rong1, GAO Na-na1, GAAWE Dorje2
1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;
2. Tibetan Traditional Medical College, Lhasa 850000, China
Abstract:
As a common Tibetan herb, Bawo Sebo was mainly used in the treatment of rheumatoid arthritis and urarthritis in Traditional Tibetan medicine. Based on our ethnobotanical survey, the origin of the herb was determined as Swertia verticillifolia T. N. Ho et S. W. Liu (Gentianaceae), endemic to the region of the Qinghai-Tibet Plateau. The diagnostic characters:perennial; stem leaves in whorls; corolla campanulate, yellow-green, 4-lobed; nectary 1 per corolla lobe, naked. Also, its complete chloroplast (cp) genome was sequenced. It is 151 682 bp in length, including a large single copy (LSC) region of 82 623 bp, a small single copy (SSC) region of 18 335 bp and a pair of inverted repeats (IRs) of 25 362 bp. It contains 129 unique genes, including 84 protein-coding genes, 37 tRNAs and 8 rRNAs. This study provides information for understanding the diversity of Swertia cp genomes, and the alpine species identification, conservation and molecular phylogenetic researches of Swertia and Gentianaceae.
Key words:   
收稿日期: 2018-02-02
DOI: 10.16438/j.0513-4870.2018-0121
基金项目: 国家自然科学基金面上项目(81173654);国家自然科学基金青年科学基金项目(81503354).
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