药学学报, 2018, 53(9): 1526-1531
引用本文:
魏华, 刘一涵, 田云刚, 向芳芳. 长果雪胆块茎中的葫芦烷三萜类化学成分研究[J]. 药学学报, 2018, 53(9): 1526-1531.
WEI Hua, LIU Yi-han, TIAN Yun-gang, XIANG Fang-fang. Cucurbitane-type triterpenoids from the tubers of Hemsleya dolichocarpa[J]. Acta Pharmaceutica Sinica, 2018, 53(9): 1526-1531.

长果雪胆块茎中的葫芦烷三萜类化学成分研究
魏华1,2, 刘一涵1, 田云刚1, 向芳芳1
1. 吉首大学 湖南省土家医药研究中心, 湖南 吉首 416000;
2. 吉首大学 植物资源保护与利用湖南省高校重点实验室, 湖南 吉首 416000
摘要:
采用硅胶、Sephadex LH-20、ODS中压色谱、半制备高效液相等色谱技术对长果雪胆块茎的乙醇提取物进行分离纯化,从中分离得到了7个葫芦烷三萜类化合物,根据理化性质和波谱数据鉴定为3β,11α,26,27-tetrahydroxycucurbita-5,24(E)-diene-3,26-glucosides(1)、scandenogenin D(2)、jinfushanencin F(3)、scandenoside R3(4)、scandenoside R15)、scandenogenin A(6)和scandenoside R27)。其中化合物1为一新化合物,体外抗肿瘤活性筛选发现化合物2对人宫颈癌细胞HeLa具有较强的抑制作用,IC50为6.78 μmol·L-1
关键词:    葫芦科      雪胆属      长果雪胆      葫芦烷三萜      抗肿瘤活性     
Cucurbitane-type triterpenoids from the tubers of Hemsleya dolichocarpa
WEI Hua1,2, LIU Yi-han1, TIAN Yun-gang1, XIANG Fang-fang1
1. Tujia Medicine Research Center in Hunan, College of Hunan Province, Jishou University, Jishou 416000, China;
2. Key Laboratory of Plant Resources Conservation and Utilization, College of Hunan Province, Jishou University, Jishou 416000, China
Abstract:
Seven cucurbitane-type triterpenoids were isolated from the ethanol extract of the tubers of Hemsleya dolichocarpa, with a combination of various chromatographic approaches, including silica gel, Sephadex LH-20, Semi-HPLC and so on. On the basis of spectroscopic data analysis, they were identified as 3β,11α,26,27-tetrahydroxycucurbita-5,24(E)-diene-3,26-glucosides (1), scandenogenin D (2), jinfushanencin F (3), scandenoside R3 (4), scandenoside R1 (5), scandenogenin A (6), scandenoside R2 (7). Among them, compound 1 is a new triterpenoid, compound 2 showed remarkable activity against human cancer cell line HeLa with IC50 value of 6.78 μmol·L-1.
Key words:    Cucurbitaceae    Hemsleya genus    H.dolichocarpa    cucurbitane-type triterpenoids    anti-tumor activity   
收稿日期: 2018-06-06
DOI: 10.16438/j.0513-4870.2018-0526
基金项目: 湖南省土家医药研究中心开放项目(20172001);植物资源保护与利用湖南省高校重点实验室开放项目(JSK2017YX008);湖南省重点学科建设项目(JSU0713);湘西州2016年科技计划一般项目.
通讯作者: 魏华,Tel:18974392218,E-mail:weihua20@126.com
Email: weihua20@126.com
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