药学学报, 2018, 53(11): 1862-1867
引用本文:
王航航, 李刚, 彭晓娉, 娄红祥. 狭叶十大功劳内生真菌Colletotrichum fioriniae F18的次级代谢产物研究[J]. 药学学报, 2018, 53(11): 1862-1867.
WANG Hang-hang, LI Gang, PENG Xiao-ping, LOU Hong-xiang. Secondary metabolites from Colletotrichum fioriniae F18, an endophytic fungus isolated from the medicinal plant Mahonia fortunei[J]. Acta Pharmaceutica Sinica, 2018, 53(11): 1862-1867.

狭叶十大功劳内生真菌Colletotrichum fioriniae F18的次级代谢产物研究
王航航1, 李刚1, 彭晓娉1, 娄红祥1,2
1. 青岛大学药学院天然药物与生药学系, 山东 青岛 266021;
2. 山东大学药学院, 山东 济南 250012
摘要:
对分离自狭叶十大功劳茎中的一株内生真菌Colletotrichum fioriniae F18进行化学成分研究,从其大米发酵产物中分离得到1个新吲哚生物碱makomotindoline B(1)和2个已知吲哚衍生物:3-indoleacetic acidmethyl ester(2)和N-acetyltryptamine(3),以及6个芳香化合物:对羟基苯乙酸(4)、对羟基苯乙醇(5)、对甲氧基苯乙酸(6)、4-hydroxyphenethyl 2-(4-hydroxyphenyl) acetate(7)、regiolone(8)和N-苯乙基乙酰胺(9)。通过质谱和核磁共振等技术确定了上述化合物的结构,并通过圆二色谱ECD量子化学计算确定了化合物1的绝对构型。抗菌活性测试结果表明,化合物1~9Bacillus subtilisStaphylococcus aureusEscherichia coliPseudomonas aeruginosa无明显抑制作用,对基因缺陷型紫色杆菌Chromobacterium violaceum也无群体感应抑制活性。
关键词:   
Secondary metabolites from Colletotrichum fioriniae F18, an endophytic fungus isolated from the medicinal plant Mahonia fortunei
WANG Hang-hang1, LI Gang1, PENG Xiao-ping1, LOU Hong-xiang1,2
1. Department of Natural Medicinal Chemistry and Pharmacognosy, School of Pharmacy, Qingdao University, Qingdao 266021, China;
2. School of Pharmacy, Shandong University, Ji'nan 250012, China
Abstract:
Chemical investigation on the rice culture of an endophytic fungus Colletotrichum fioriniae F18, inhabiting in the stems of the medicinal plant Mahonia fortunei, led to the isolation of nine compounds. They included a new indole alkaloid, makomotindoline B (1), and two known indole derivatives, 3-indoleacetic acid methyl ester (2) and N-acetyltryptamine (3), together with six known aromatic compounds, 2-(4-hydroxyphenyl) acetic acid (4), 4-(2-hydroxyethyl)phenol (5), 2-(4-methoxyphenyl)acetic acid (6), 4-hydroxyphenethyl 2-(4-hydroxyphenyl)acetate (7), regiolone (8) and N-phenethylacetamide (9). The structures of these compounds were elucidated based on the analysis of spectroscopic data including MS and NMR. The absolute configuretion of compound 1 was determined by electronic circular dichroism (ECD) calculation. Antibacterial activity assay indicated that compounds 1-9 had no antibacterial activities against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, as well as no quorum sensing inhibitory (QSI) activity for Chromobacterium violaceum.
Key words:   
收稿日期: 2018-09-14
DOI: 10.16438/j.0513-4870.2018-0838
基金项目: 青岛大学科研启动经费项目(29016010007010).
通讯作者: 娄红祥,Tel/Fax:86-531-8838-2012,E-mail:louhongxiang@sdu.edu.cn
Email: louhongxiang@sdu.edu.cn
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