药学学报, 2019, 54(1): 130-137
引用本文:
邹叶廷, 徐金娣, 龙芳, 张业清, 李松林. 整合UPLC-QTOF-MS/MS全扫描和模拟MRM方法综合评价茯苓乙醇提取物与后续乙酸乙酯萃取物三萜酸类组分化学一致性[J]. 药学学报, 2019, 54(1): 130-137.
ZOU Ye-ting, XU Jin-di, LONG Fang, ZHANG Ye-qing, LI Song-lin. Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode[J]. Acta Pharmaceutica Sinica, 2019, 54(1): 130-137.

整合UPLC-QTOF-MS/MS全扫描和模拟MRM方法综合评价茯苓乙醇提取物与后续乙酸乙酯萃取物三萜酸类组分化学一致性
邹叶廷1,2, 徐金娣2, 龙芳1,3, 张业清3, 李松林1,2
1. 南京中医药大学附属中西医结合医院中药质量研究室, 江苏 南京 210028;
2. 江苏省中医药研究院和中国中医科学院江苏分院中药代谢组研究室, 江苏 南京 210028;
3. 南京中医药大学附属中西医结合医院呼吸科, 江苏 南京 210028
摘要:
本研究采用超高效液相色谱串联四级杆飞行时间质谱法(UPLC-QTOF-MS/MS)对茯苓乙醇提取物与后续乙酸乙酯萃取物中三萜酸类成分的化学一致性进行评价。通过全扫描高分辨质谱数据分析,对茯苓的乙醇提取物和后续乙酸乙酯萃取物中的化学组成进行定性比较,结合文献及对照品数据对照,共鉴定出23个主要三萜酸类成分。利用模拟三重四级杆质谱(TQ-MS)的多反应监控(mMRM)模式对上述23个主要成分进行定量或相对定量分析,并计算三萜酸类组分在萃取过程中的转移率和富集率。结果表明,乙酸乙酯萃取物与乙醇提取物化学组成基本一致,萃取过程中三萜酸类成分转移率高,通过萃取可有效富集三萜酸类组分。本研究不但为科学评估后续乙酸乙酯萃取茯苓三萜酸类组分的必要性提供依据,而且为综合评价中药提取物在萃取前后的化学一致性提供了参考方法。
关键词:    茯苓      三萜酸      萃取      化学一致性      UPLC-QTOF-MS/MS     
Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode
ZOU Ye-ting1,2, XU Jin-di2, LONG Fang1,3, ZHANG Ye-qing3, LI Song-lin1,2
1. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China;
2. Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of Chinese Academy of Traditional Chinese Medicine, Nanjing 210028, China;
3. Department of Respiratory Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
Abstract:
An ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) method was developed to evaluate the chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof. First, high resolution mass spectrometry data were obtained with Full scan mode, by comparing with MS data from the reference compounds and literatures, a total of 23 components were unequivocally or tentatively identified in ethanol extracts and acetic ether extracts thereof. Then, a mimic multiple reaction monitoring (mMRM) mode was established using UPLC-QTOF-MS/MS to quantify the triterpene acids in ethanol extracts and acetic ether extracts thereof. Eleven components were absolutely quantified with reference compounds, while 12 components without reference compounds were relatively quantified with peak areas, the transfer and enrichment rate of triterpene acids during liquid-liquid extraction were calculated. It was found all of the 23 triterpene acids identified in Poria ethanol extracts could be transferred into acetic ether extracts with high transfer and enrichment rate. The present study provides not only scientific evidence for further extraction of triterpene acids in Poria by acetic ether, but also an approach for comprehensive evaluation of the chemical consistency of herbal medicine extracts before and after the liquid-liquid extraction.
Key words:    Poria    triterpene acid    liquid-liquid extraction    chemical consistency    UPLC-QTOF-MS/MS   
收稿日期: 2018-07-23
DOI: 10.16438/j.0513-4870.2018-0670
基金项目: 国家自然科学基金项目(81603262);江苏省自然科学基金项目(BK20161081);江苏省中医药局项目(LZ11066).
通讯作者: 李松林,Tel:86-25-85639640,E-mail:songlinli64@126.com
Email: songlinli64@126.com
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