药学学报, 2019, 54(1): 144-150
杨瑞, 钱文娟, 彭琳秀, 徐佳, 谢彤, 纪建建, 詹秀琴, 单进军. 基于UHPLC-Q-Exactive Orbitrap/MS的桔梗汤治疗小鼠急性肺损伤的磷脂组学研究[J]. 药学学报, 2019, 54(1): 144-150.
YANG Rui, QIAN Wen-juan, PENG Lin-xiu, XU Jia, XIE Tong, JI Jian-jian, ZHAN Xiu-qin, SHAN Jin-jun. Phospholipidomics study of Jiegeng Decotion for LPS-induced acute lung injury in mice based on UHPLC-Q-Exactive Orbitrap/MS[J]. Acta Pharmaceutica Sinica, 2019, 54(1): 144-150.

基于UHPLC-Q-Exactive Orbitrap/MS的桔梗汤治疗小鼠急性肺损伤的磷脂组学研究
杨瑞1,2, 钱文娟2,3, 彭琳秀2,3, 徐佳2, 谢彤2, 纪建建2, 詹秀琴1, 单进军1,2,3
1. 南京中医药大学医学与生命科学学院, 江苏省儿童呼吸疾病(中医药)重点实验室, 江苏 南京 210023;
2. 南京中医药大学中医儿科学研究所, 江苏 南京 210023;
3. 南京中医药大学药学院, 江苏省中药高效给药系统工程技术研究中心, 江苏 南京 210023
采用基于UHPLC-Q-Exactive Orbitrap/MS的脂质组学方法,分析脂多糖(lipopolysaccharide,LPS)诱导的急性肺损伤(acute lung injury,ALI)模型小鼠的肺组织磷脂代谢的变化,观察桔梗汤对异常脂质的调节,探究桔梗汤对LPS诱导的ALI的调节作用。分别采集空白对照组、ALI模型组、地塞米松(阳性药)组、桔梗汤组小鼠的肺组织样本,实验方案经南京中医药大学实验动物伦理委员会批准实施,提取其脂质成分,采用UHPLC-Q-Exactive Orbitrap/MS脂质组学技术研究各组肺组织磷脂的变化。LPS诱导的ALI小鼠磷脂出现代谢异常,具体表现为磷脂酰胆碱(PC)类脂质出现明显的上调,磷脂酰乙醇胺(PE)、磷脂酰甘油(PG)、磷脂酰丝氨酸(PS)、磷脂酰肌醇(PI)等脂质出现代谢紊乱,桔梗汤对这些变化磷脂具有一定的回调作用。LPS诱导的ALI引起体内磷脂紊乱,桔梗汤对代谢紊乱的磷脂具有调控作用。
关键词:    UHPLC-Q-Exactive Orbitrap/MS      脂多糖      急性肺损伤      桔梗汤      磷脂组学     
Phospholipidomics study of Jiegeng Decotion for LPS-induced acute lung injury in mice based on UHPLC-Q-Exactive Orbitrap/MS
YANG Rui1,2, QIAN Wen-juan2,3, PENG Lin-xiu2,3, XU Jia2, XIE Tong2, JI Jian-jian2, ZHAN Xiu-qin1, SHAN Jin-jun1,2,3
1. Jiangsu Key Laboratory of Pediatric Respiratory Disease, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China;
2. Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;
3. College of Pharmacy, Jiangsu Provincial TCM Engineering Technology Research Center of High Efficient Drug Delivery System DDS, Nanjing University of Chinese Medicine, Nanjing 210023, China
Using the lipidomics method based on UHPLC-Q-Exactive Orbitrap/MS, the change of phospholipid metabolism in lung tissue of mice induced by lipopolysaccharide (LPS)-induced acute lung injury was analyzed to observe the regulation of abnormal lipids by Jiegeng Decoction and to explore the regulation effect of Jiegeng Decoction on LPS-induced acute lung injury. The lung tissue samples from control group, model group, dexamethasone (positive drug) group, and Jiegeng Decoction group were collected and the lipid components of the sample were extracted. All procedures over mice were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Nanjing University of Chinese Medicine, and the experiments were approved by the Animal Ethics Committee of our university. The lipidomics technique of UHPLC-Q-Exactive Orbitrap/MS was used to study change of phospholipids in lung tissue of each group. LPS induced acute lung injury in mice with metabolic abnormalities of phospholipids, the specific performance of the PC was significantly upregulated, phosphatidyl ethanolamine (PE), phosphatidyl glycerol (PG), phosphatidyl serine (PS),phosphatidylinositol (PI) and other metabolic disorders, Jiegeng Decoction have a certain role in these phospholipids. LPS-induced acute lung injury caused disturbances of phospholipid in vivo, and Jiegeng Decoction regulates metabolic phospholipids.
Key words:    UHPLC-Q-Exactive Orbitrap/MS    lipopolysaccharide    acute lung injury    Jiegeng Decoction    phospholipidomics   
收稿日期: 2018-06-30
DOI: 10.16438/j.0513-4870.2018-0598
基金项目: 国家自然科学基金资助项目(81774156);江苏省自然科学基金资助项目(BK20161573);江苏省第十四批"六大人才高峰"高层次人才选拔培养项目(YY-022);江苏省"333工程"科研项目(BRA2016427).
通讯作者: 詹秀琴,Tel:13951631349,E-mail:1543641179@qq.com;单进军,Tel:86-25-85811329,E-mail:dfsjj@163.com
Email: 1543641179@qq.com;dfsjj@163.com
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