药学学报, 2019, 54(3): 533-539
引用本文:
宋佳, 庞溢媛, 高丽, 秦雪梅, 杜冠华, 周玉枝. 基于血清代谢组学的黄芩花干预D-半乳糖致衰老大鼠作用研究[J]. 药学学报, 2019, 54(3): 533-539.
SONG Jia, PANG Yi-yuan, GAO Li, QIN Xue-mei, DU Guan-hua, ZHOU Yu-zhi. Effects of Scutellaria baicalensis Georgi flowers on D-galactose induced aging in rats based on serum metabolomics[J]. Acta Pharmaceutica Sinica, 2019, 54(3): 533-539.

基于血清代谢组学的黄芩花干预D-半乳糖致衰老大鼠作用研究
宋佳1,2, 庞溢媛1, 高丽1, 秦雪梅1, 杜冠华1,3, 周玉枝1
1. 山西大学中医药现代研究中心, 山西 太原 030006;
2. 山西大学化学化工学院, 山西 太原 030006;
3. 中国医学科学院药物研究所, 北京 100050
摘要:
研究黄芩花提取物(Scutellaria flower extract,SFE)体外抗氧化能力与对D-半乳糖致衰老大鼠的保护作用及机制。以羟基自由基和超氧阴离子自由基的清除能力为指标评价SFE体外抗氧化能力。采用D-半乳糖致衰老大鼠模型,造模的同时灌胃给予不同剂量的SFE。观察大鼠自主活动能力并通过代谢组学技术结合多元统计方法对大鼠血清进行分析,探讨SFE抗衰老作用及机制,并对其主要代谢通路谷氨酰胺和谷氨酸代谢进行定量验证。动物实验所有程序均获得山西大学伦理委员会批准。结果表明,SFE具有体外抗氧化能力,能够改善衰老大鼠行为。通过代谢组学技术找到与衰老相关的9个潜在生物标志物,共涉及7条代谢通路,给予高剂量SFE干预后生物标志物均出现回调。
关键词:   
Effects of Scutellaria baicalensis Georgi flowers on D-galactose induced aging in rats based on serum metabolomics
SONG Jia1,2, PANG Yi-yuan1, GAO Li1, QIN Xue-mei1, DU Guan-hua1,3, ZHOU Yu-zhi1
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
3. Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China
Abstract:
This study aimed to evaluate the antioxidant activities of Scutellaria baicalensis Georgi flowers by measuring the scavenging activity of hydroxyl radical and superoxide anion radical. Aging of rats was induced by D-galactose and protective effects of Scutellaria flower extract (SFE) at different doses were evaluated by locomotor activities. To explore the mechanism of SFE, metabolomic technique combined with multivariate statistical methods was used to analyze the profile of metabolites from rat serum and quantify the levels of glutamine and glutamate in the serum. All research procedures were approved according to the Committee on the Ethics of Animal Experiments of Shanxi University. The results showed that high-dose of SFE had antioxidant activity in vitro, and could ameliorate the aging behavior of rats. A total of 9 potential biomarkers related to aging were found, pointing to the involvement of 7 metabolic pathways.
Key words:   
收稿日期: 2018-10-11
DOI: 10.16438/j.0513-4870.2018-0927
基金项目: 山西省重点研发计划项目(201703D321023-3);山西省科技基础条件平台建设项目(2014091022);山西省科技攻关项目(20140313008-14);山西省重点技术创新项目[晋财建-(2013)99号].
通讯作者: 周玉枝
Email: zhouyuzhi@sxu.edu.cn
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