药学学报, 2019, 54(4): 720-728
引用本文:
朱十伟, 宫文霞, 陈聪聪, 阴奇材, 李肖, 秦雪梅, 杜冠华, 周玉枝. 基于1H NMR代谢组学技术的柴胡-白芍药对对逍遥散的贡献研究[J]. 药学学报, 2019, 54(4): 720-728.
ZHU Shi-wei, GONG Wen-xia, CHEN Cong-cong, YIN Qi-cai, LI Xiao, QIN Xue-mei, DU Guan-hua, ZHOU Yu-zhi. The contribution of Radix Bupleuri-Radix Paeoniae Alba herb pair to Xiaoyaosan based on 1H NMR metabolomics[J]. Acta Pharmaceutica Sinica, 2019, 54(4): 720-728.

基于1H NMR代谢组学技术的柴胡-白芍药对对逍遥散的贡献研究
朱十伟1,2, 宫文霞1, 陈聪聪1,2, 阴奇材1,2, 李肖1, 秦雪梅1, 杜冠华1,3, 周玉枝1
1. 山西大学中医药现代研究中心, 山西 太原 030006;
2. 山西大学化学化工学院, 山西 太原 030006;
3. 中国医学科学院、北京协和医学院药物研究所, 北京 100050
摘要:
探究柴胡-白芍药对在逍遥散抗抑郁作用中的核心地位及作用机制。通过行为学指标观察柴胡-白芍药对、逍遥散、阴性对照(逍遥散除去柴胡、白芍)对慢性温和不可预知性抑郁(CUMS)模型大鼠行为学的影响,结果显示模型组大鼠体重、穿越格数和直立次数、糖水偏爱率均显著下降(P<0.001),强迫游泳不动时间明显延长(P<0.01)。柴胡-白芍药对有明显的抗抑郁作用,且低剂量组对大鼠穿越格数回调与逍遥散相当,高剂量组对直立次数和糖水偏爱率回调与逍遥散相当;高、低剂量组对不动时间回调均优于逍遥散。动物实验获得山西大学伦理委员会的批准。代谢组学结果提示,与抑郁症相关的15种生物标志物中,其中逍遥散回调12种,药对回调8种,阴性对照仅回调6种。通过代谢通路分析,共聚焦了4条抑郁症相关代谢通路,药对和逍遥散通过4条相同的代谢通路发挥抗抑郁作用,阴性对照仅通过其中3条通路发挥作用。综上,从行为学、代谢组学和通路分析揭示了柴胡-白芍药对在逍遥散抗抑郁中发挥重要作用。
关键词:    代谢组学      柴胡      白芍      药对      逍遥散      抗抑郁     
The contribution of Radix Bupleuri-Radix Paeoniae Alba herb pair to Xiaoyaosan based on 1H NMR metabolomics
ZHU Shi-wei1,2, GONG Wen-xia1, CHEN Cong-cong1,2, YIN Qi-cai1,2, LI Xiao1, QIN Xue-mei1, DU Guan-hua1,3, ZHOU Yu-zhi1
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
3. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Abstract:
This study explores the antidepressant mechanism of Radix Bupleuri-Radix Paeoniae Alba herb pair and the core role in Xiaoyaosan. The behavioral indicators were used to observe the effects of herb pair, Xiaoyaosan, and a negative control (Xiaoyaosan remove Radix Bupleuri-Radix Paeoniae Alba) on CUMS rats. The body weight, number of crossing and rearing, sugar preference rate were significantly decreased (P<0.001) and the immobility time of forced swimming test was significantly prolonged (P<0.01) in the model group. Radix Bupleuri-Radix Paeoniae Alba herb pair has significant antidepressant effect, with the number of crossing in low dose group similar to Xiaoyaosan, while the number of rearing and the sucrose preference rate in high dose group are equivalent to Xiaoyaosan, and the immobility time in high and low-doses group are better than Xiaoyaosan. Animal experimentation was approved according to the Committee on the Ethics of Animal Experiments of Shanxi University. Metabonomics results showed 15 biomarkers related to depression, whereas administering Xiaoyaosan or the herb pair can ameliorate 12 or 8 metabolites respectively, compared to the negative control, which can only ameliorate 6 metabolites. The metabolic pathway analysis showed 5 depression-related pathways. The herb pair and Xiaoyaosan exerted antidepressant effects through common 4 metabolic pathways, while the negative control only exerted effects through 3. In summary, behavioral, metabolomics and metabolic pathway analysis revealed that Radix Bupleuri-Radix Paeoniae Alba plays an important role in the biological effect of Xiaoyaosan.
Key words:    metabolomics    Radix Bupleuri    Radix Paeoniae Alba    herb pair    Xiaoyaosan    antidepressant   
收稿日期: 2018-12-18
DOI: 10.16438/j.0513-4870.2018-1123
基金项目: 国家自然科学基金面上项目(81673572);山西省应用基础研究项目(201601D021164);山西省高校科技创新项目(2016120).
通讯作者: 周玉枝
Email: zhouyuzhi@sxu.edu.cn
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