药学学报, 2019, 54(5): 867-876
张立雯, 宿树兰, 戴新新, 魏丹丹, 朱悦, 钱大玮, 段金廒. 桑叶有效组分对db/db小鼠肠道菌群的调节作用[J]. 药学学报, 2019, 54(5): 867-876.
ZHANG Li-wen, SU Shu-lan, DAI Xin-xin, WEI Dan-dan, ZHU Yue, QIAN Da-wei, DUAN Jin-ao. Regulatory effect of mulberry leaf components on intestinal microflora in db/db mice[J]. Acta Pharmaceutica Sinica, 2019, 54(5): 867-876.

张立雯, 宿树兰, 戴新新, 魏丹丹, 朱悦, 钱大玮, 段金廒
南京中医药大学, 江苏省中药资源产业化过程协同创新中心, 中药资源产业化与方剂创新药物国家地方联合工程研究中心, 国家中医药管理局中药资源循环利用重点研究室, 江苏 南京 210023
以自发性肥胖的糖尿病模型db/db小鼠为研究对象,在药效评价的基础上,通过16S rDNA测序的方法研究桑叶黄酮、多糖、生物碱对糖尿病小鼠的生物效应及其对肠道菌群的调节作用。动物实验通过了南京中医药大学伦理委员会审查,10只db/m小鼠作为空白组(control),40只db/db小鼠随机分为模型组(model)、二甲双胍组(metformin)、桑叶黄酮组(MF)、桑叶多糖组(MP)、桑叶生物碱组(MA),灌胃给药6周。结果表明,与正常组相比,db/db小鼠肠道内菌群从门水平到属水平均发生了显著变化;且模型组小鼠肠道菌中厚壁菌门、变形菌门等比例显著降低,拟杆菌的比例升高;给药后拟杆菌、厚壁菌门中的毛螺菌科、罗斯氏菌属以及脱硫杆菌属等均得到有效调节,尤其是生物碱组调节作用最为显著。这提示桑叶有效组分具有改善db/db小鼠肠道菌群失调的作用。
Regulatory effect of mulberry leaf components on intestinal microflora in db/db mice
ZHANG Li-wen, SU Shu-lan, DAI Xin-xin, WEI Dan-dan, ZHU Yue, QIAN Da-wei, DUAN Jin-ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing 210023, China
The 16S rDNA sequencing method was adopted to study the effects of mulberry leaf flavonoids, polysaccharides and alkaloids on intestinal microflora in db/db diabetic mice. The animal experiment was examined by the Ethics Committee of Nanjing University of Chinese Medicine. Ten db/m mice were control group and forty db/db mice were randomly divided into model group, metformin group, mulberry flavonoid (MF) group, mulberry polysaccharide (MP) group and mulberry alkaloid (MA) group. After intragastric administration for six weeks, fresh feces were collected for detection of intestinal microflora. There were Firmicutes, Bacteroidetes, Proteobacteria, Saccharibacteria, Tenericutes, Deferribacteres, Verrucomicrobia, Cyanobacteria in each group. The results showed that the intestinal microflora of db/db mice changed significantly from phylum level to genus level. The proportion of Firmicutes and Proteobacteria in model group decreased significantly, and the proportion of Bacteroidetes increased. The difference in species abundance distribution between model group and other groups was significant, which indicated that the community distribution was disordered in model group. After administration, the Bacteroidetes, Lachnospiraceae, Roseburia and Desulfovibrio were effectively regulated, especially in the alkaloid group. The difference in species abundance distribution between drug-treated group and blank group also became smaller. It is suggested that the active components of mulberry leaf have the effect of improving the intestinal microflora imbalance in db/db mice.
Key words:   
收稿日期: 2018-10-11
DOI: 10.16438/j.0513-4870.2018-0932
基金项目: 国家自然科学基金面上项目(81373889,81673533);江苏省中药资源产业化过程协同创新中心重点项目资助(ZDXM-10).
通讯作者: 宿树兰,Tel/Fax:86-25-85811917,E-mail:sushulan1974@163.com;段金廒,E-mail:dja@njucm.edu.cn
Email: sushulan1974@163.com;dja@njucm.edu.cn
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