药学学报, 2019, 54(5): 906-912
引用本文:
夏琪涵, 郭明, 侯平, 顾奕, 赵富荣. 典型生物碱与血清蛋白相互作用CE分析方法比较与理论建模研究[J]. 药学学报, 2019, 54(5): 906-912.
XIA Qi-han, GUO Ming, HOU Ping, GU Yi, ZHAO Fu-rong. Comparison and theoretical modeling of capillary electrophoresis analysis for the interaction between caffeine and serum proteins[J]. Acta Pharmaceutica Sinica, 2019, 54(5): 906-912.

典型生物碱与血清蛋白相互作用CE分析方法比较与理论建模研究
夏琪涵1, 郭明1,2, 侯平1, 顾奕1, 赵富荣1
1. 浙江农林大学林业与生物技术学院, 浙江 杭州 311300;
2. 浙江农林大学理学院, 浙江 杭州 311300
摘要:
利用毛细管电泳技术建立咖啡因(caffeine)和人血清白蛋白(HSA)相互作用的分析方法。在生理条件下建立配体-受体相互作用模型(caffeine-HSA),通过前沿分析(frontal-analysis,FA)法、简化Hummel-Dreyer(HD)法、区段-区段动力学(plug-plug kinetic,PPK)法测定两者间的相互作用。运用非线性方程、Scatchard方程、Klotz方程获得caffeine-HSA的相互作用参数,进而分析理论模型适用度。结果表明:FA法、HD法、PPK法均适用于caffeineHSA相互作用体系的分析,其中HD法最优,模型适用度分析得出非线性回归方程为最适理论模型。相互作用参数测试表明,caffeine-HSA相互作用体系之间发生的结合为单一类型的结合位点且结合稳定强度适中。相关工作阐明了caffeine-HSA相互作用的机制,可为典型生物碱的深入研究提供有益参考。
关键词:    生物碱      咖啡因      人血清白蛋白      理论模型      相互作用参数     
Comparison and theoretical modeling of capillary electrophoresis analysis for the interaction between caffeine and serum proteins
XIA Qi-han1, GUO Ming1,2, HOU Ping1, GU Yi1, ZHAO Fu-rong1
1. School of Forestry and Bio-technology, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China;
2. School of Science, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
Abstract:
The method for analyzing the interaction between caffeine and human serum albumin (HSA) was established by capillary electrophoresis. Under physiological conditions, the interaction between ligand (caffeine)-receptor (HSA) was studied with frontier-analysis (FA) method, Hummel-Dreyer (HD) method and plug-plug kinetic (PPK) method. The interaction parameters of caffeine-HSA system were obtained using non-linear equation, Scatchard equation and Klotz equation. The results showed that FA, HD and PPK methods were suitable for caffeine-HSA system, among them, HD method was the best, and the Non-linear equation was the best theoretical model to caffeine-HSA system. Interaction parameter tests showed that caffeine-HSA interaction was a single site interaction and the binding stability was moderate. The mechanism of caffeine-HSA interaction has been elucidated, which can provide valuable information for further research of alkaloids.
Key words:    alkaloid    caffeine    human serum albumin    theoretical model    interaction parameter   
收稿日期: 2018-12-25
DOI: 10.16438/j.0513-4870.2018-1146
基金项目: 国家自然科学基金资助项目(20877072);浙江省省院合作科技项目资助(2015SY11).
通讯作者: 郭明,Tel:86-571-61075662,Fax:86-571-63732772,E-mail:guoming@zafu.edu.cn
Email: guoming@zafu.edu.cn
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