药学学报, 2019, 54(6): 1036-1040
引用本文:
王玲, 李江, 徐少锋, 冯楠, 章菽, 王晓良. 人工麝香对大鼠急性脑缺血再灌损伤和脑出血的实验治疗[J]. 药学学报, 2019, 54(6): 1036-1040.
WANG Ling, LI Jiang, XU Shao-feng, FENG Nan, ZHANG Shu, WANG Xiao-liang. Therapeutic effects of artificial musk on acute ischemic stroke and subarachnoid hemorrhage in rats[J]. Acta Pharmaceutica Sinica, 2019, 54(6): 1036-1040.

人工麝香对大鼠急性脑缺血再灌损伤和脑出血的实验治疗
王玲1, 李江1, 徐少锋1, 冯楠1, 章菽2, 王晓良1
1. 中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050;
2. 北京联馨药业有限公司, 北京 102628
摘要:
采用大鼠局灶性脑缺血再灌注和蛛网膜下腔出血的动物模型,评价人工麝香对缺血性脑卒中和出血性脑卒中的治疗作用,为人工麝香的临床应用提供研究基础。所有动物实验都遵循北京协和医学院动物伦理委员会的规定。结果显示,大脑中动脉阻断或蛛网膜下腔出血5 min后灌胃给予人工麝香对急性缺血性和出血性脑卒中均具有明显的保护作用。在10~200 mg·kg-1剂量范围内,对模型大鼠的死亡率、神经行为学和脑梗死体积具有一定的量效关系。其中在缺血性脑卒中,人工麝香的有效剂量为10 mg·kg-1;在出血性脑卒中,其有效剂量为200 mg·kg-1。上述研究结果提示人工麝香在治疗缺血性脑卒中和出血性脑卒中时存在一定差别,临床使用中要给予区别。
关键词:    人工麝香      脑卒中      脑缺血再灌注      蛛网膜下腔出血      大脑中动脉梗塞     
Therapeutic effects of artificial musk on acute ischemic stroke and subarachnoid hemorrhage in rats
WANG Ling1, LI Jiang1, XU Shao-feng1, FENG Nan1, ZHANG Shu2, WANG Xiao-liang1
1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Natural Products and Functions, Beijing 100050, China;
2. Beijing Lianxin Pharmaceutical Co., Ltd., Beijing 102628, China
Abstract:
The rat models of focal cerebral ischemic reperfusion and subarachnoid hemorrhage were used to evaluate the therapeutic effects of artificial musk to provide support for its clinical application. All animal experiments were performed following the regulations of the Animal Ethics Committee of Peking Union Medical College. The results showed that oral administration of artificial musk had significant protective effects on acute ischemic and hemorrhagic stroke. In the dose range of 10-200 mg·kg-1, the mortality, neurobehavioral and cerebral infarction volume of rats in model group indicated a clear dose dependent relationship. The effective dose of artificial musk is 10 mg·kg-1 in ischemic stroke and 200 mg·kg-1 in hemorrhagic stroke. These findings suggest that the treatments of artificial musk in ischemic stroke and in hemorrhagic stroke are different, and such differences should be noted for its clinical use.
Key words:    artificial musk    stroke    cerebral ischemia-reperfusion    subarachnoid hemorrhage    middle cerebral artery occlusion   
收稿日期: 2019-03-22
DOI: 10.16438/j.0513-4870.2019-0198
基金项目: 国家“重大新药创制”科技重大专项资助项目(2012ZX09301002-004);新药作用机制研究与药效评价北京市重点实验室(BZ0150).
通讯作者: 王晓良
Email: wangxl@imm.ac.cn
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