药学学报, 2019, 54(6): 1082-1087
引用本文:
姜雨彤, 高照, 乐智勇, 白宗利, 屠鹏飞, 姜勇. 不同等级制远志饮片的质量分析[J]. 药学学报, 2019, 54(6): 1082-1087.
JIANG Yu-tong, GAO Zhao, LE Zhi-yong, BAI Zong-li, TU Peng-fei, JIANG Yong. Quality analysis of different grades of Glycyrrhiza Polygalae Radix slices[J]. Acta Pharmaceutica Sinica, 2019, 54(6): 1082-1087.

不同等级制远志饮片的质量分析
姜雨彤1, 高照1, 乐智勇2, 白宗利2, 屠鹏飞1, 姜勇1
1. 北京大学药学院天然药物及仿生药物国家重点实验室, 北京 100191;
2. 康美药业股份有限公司, 广东 普宁 515300
摘要:
建立不同制远志饮片商品等级的划分和质量分析方法,并对不同等级的制远志进行分析及比较,为其等级标准的制定提供参考。收集12家饮片企业的制远志样品共34批,根据其外观直径大小,将制远志分为两个等级:一等品(直径≥ 3.0 mm)、二等品(直径<3.0 mm)。采用薄层色谱、含量测定和指纹图谱分析方法对不同等级的制远志样品进行分析,采用偏最小二乘法-判别分析(PLS-DA)和正交偏最小二乘法-判别分析(OPLS-DA)对不同等级制远志的指纹图谱进行统计分析。结果显示,建立的薄层色谱法可以同时鉴别制远志中的三大类主要成分:糖酯、(口山)酮和皂苷类,信息量丰富,成本低廉,操作简便安全,相比现有方法具有明显优势。指标性成分含量测定结果显示制远志一等品中的远志(口山)酮Ⅲ、3,6'-二芥子酰基蔗糖和细叶远志皂苷的含量均低于二等品;指纹图谱PLS-DA和OPLS-DA分析结果表明一等品和二等品之间有显著差异,差异的成分有sibiricose A5、sibiricose A6、远志(口山)酮Ⅲ、3,6'-二芥子酰基蔗糖和tenuifoliside A等成分。
关键词:    制远志      商品等级      薄层鉴别      含量测定      指纹图谱      PLS-DA      OPLS-DA     
Quality analysis of different grades of Glycyrrhiza Polygalae Radix slices
JIANG Yu-tong1, GAO Zhao1, LE Zhi-yong2, BAI Zong-li2, TU Peng-fei1, JIANG Yong1
1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;
2. Kangmei Pharmaceutical Co., Ltd., Puning 515300, China
Abstract:
The grading and quality analysis methods for different commercial Glycyrrhiza Polygalae Radix slices were established. The qualities of different grade samples were analyzed and compared, in order to provide useful information for the formulation of the grading standards of Glycyrrhiza Polygalae Radix slices. A total of 34 batches of Glycyrrhiza Polygalae Radix slice samples collected from 12 companies were divided into two grades:first-grade (diameter ≥ 3.0 mm) and second-grade (diameter < 3.0 mm). Thin-layer chromatography (TLC), multi-component content determination and fingerprint analysis were used to analyze the qualities of different grades of Glycyrrhiza Polygalae Radix slices, and the fingerprints were statistically analyzed using partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). The results showed that the established TLC method can simultaneously identify three major types of components, including sugar esters, xanthones, and saponins in Glycyrrhiza Polygalae Radix slices, and has obvious advantage compared to the existing methods for its rich information, low cost, and easy or safe operation. The multi-component determination showed that the contents of three index components (polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose and tenuifolin) in the first-grade products of Glycyrrhiza Polygalae Radix slices were lower than those in the second-grade products. The results of PLS-DA and OPLS-DA indicated significant differences were observed between the first-grade and second-grade products, with sibiricose A5, sibiricose A6, polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose and tenuifoliside A being identifies as the major differentiate markers.
Key words:    Glycyrrhiza Polygalae Radix slices    commodity grade    TLC    content determination    fingerprint    PLS-DA    OPLS-DA   
收稿日期: 2019-01-11
DOI: 10.16438/j.0513-4870.2019-0039
基金项目: 国家中药标准化项目(ZYBZH-Y-GD-13)——远志中药标准化建设(ZYY-2017-152);国家重点研发计划(2018YFC1707904,2018YFC1707300).
通讯作者: 姜勇
Email: yongjiang@bjmu.edu.cn
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