药学学报, 2019, 54(6): 1132-1140
贾鑫磊, 何贝轩, 郭丹丹, 高越, 郭美丽. 红花花冠伸长相关基因CtXTH1的特征与功能研究[J]. 药学学报, 2019, 54(6): 1132-1140.
JIA Xin-lei, HE Bei-xuan, GUO Dan-dan, GAO Yue, GUO Mei-li. The characteristics and functions of CtXTH1: the gene boosts the corolla elongation in safflower[J]. Acta Pharmaceutica Sinica, 2019, 54(6): 1132-1140.

贾鑫磊1,2, 何贝轩2, 郭丹丹2, 高越2, 郭美丽1,2
1. 福建中医药大学药学院, 福建 福州 350122;
2. 海军军医大学药学院, 上海 200433
本研究从红花花冠转录组数据库筛选出13个木葡聚糖内切转葡糖基/水解酶基因(XTHs)和8个膨胀素基因(EXPs)。通过花冠表达谱芯片数据和花冠长度的相关分析和qRT-PCR确认,筛选出可能与花冠伸长相关的4个XTHs和1个EXPr ≥ 0.60),分别为CtXTH1CtXTH2CtXTH3CtXTH4CtEXP1。采用RACE法克隆了这5个基因的全长序列,生物信息学分析发现CtXTH1可能与花冠发育有关,表达模式分析发现其在花中特异性积累。通过构建红花过表达重组载体(pMT39-CtXTH1)进行遗传转化发现,CtXTH1的过表达可以显著增加红花花冠长度(约5.34%~10.25%)和花冠重量(约30.00%~36.02%),同时,过表达植株种子重量、每果球小花数和种子数均有增大的趋势,而对花冠中的主要黄酮类成分的含量没有显著影响。对花冠显微结构观察发现,过表达植株在花冠管状部分表现出更松散和不规则的特征,提示CtXTH1可能有助于增加组织的松弛从而促进花冠伸长。本研究为高产红花品种的选育提供重要的参考价值。
关键词:    红花      CtXTH1      花冠伸长      转基因     
The characteristics and functions of CtXTH1: the gene boosts the corolla elongation in safflower
JIA Xin-lei1,2, HE Bei-xuan2, GUO Dan-dan2, GAO Yue2, GUO Mei-li1,2
1. Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;
2. Pharmacy College, Second Military Medical University, Shanghai 200433, China
In this study, 13 xyloglucan endotransglycosylases/hydrolases (XTHs) and 8 expansin (EXPs) were screened from safflower floret transcriptome database. Through correlation analysis between the safflower gene expression profile chip and the corolla development, only 4 XTHs (CtXTH1-4) and 1 EXP (CtEXP1) have positive relevance with corolla elongation (r ≥ 0.60) and were therefore validated by qRT-PCR. The full length of these genes were cloned by RACE. According to the bioinformatic analysis, CtXTH1 correlated with the development of the floret, and the expression pattern analysis indicated that CtXTH1 had accumulated in the floret. The recombinant vector (pMT39-CtXTH1) was constructed for gene transformation. Overexpression of CtXTH1 significantly increased the corolla length (about 5.34% to 10.25%) and corolla weight (about 30.00% to 36.02%) in transgenic safflower. The overexpression lines also showed an increasing tendency in the weight of seeds, average number of corollas per cone and average number of seeds in each cone. Meanwhile, overexpression of CtXTH1 had no significant effect on flavonoids. According to the corolla microstructure, the OVX-line tubular part of floret exhibited a looser and irregular character. These data suggested that CtXTH1 can potentially increase relaxation of the tissues and boost corolla elongation. Our study provides a valuable clue for plant breeding in the future.
Key words:    safflower    CtXTH1    corolla length    transgenic   
收稿日期: 2019-02-25
DOI: 10.16438/j.0513-4870.2019-0130
基金项目: 国家自然科学基金资助项目(81473300,81173484);上海市自然基金资助项目(13ZR1448200);“863”国家高技术发展计划(2008AA02Z137).
通讯作者: 高越, 郭美丽
Email: mlguo@126.com;gaoyue2000@hotmail.com
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贾鑫磊  在本刊中的所有文章
何贝轩  在本刊中的所有文章
郭丹丹  在本刊中的所有文章
高越  在本刊中的所有文章
郭美丽  在本刊中的所有文章

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