药学学报, 2019, 54(7): 1260-1264
引用本文:
吴云秋, 黄云峰, 罗迪, 邱莉, 谢集照, 徐焕基, 武鑫铎, 李哲明. 台东荚蒾中一个新的齐墩果烷型三萜[J]. 药学学报, 2019, 54(7): 1260-1264.
WU Yun-qiu, HUANG Yun-feng, LUO Di, QIU Li, XIE Ji-zhao, XU Huan-ji, WU Xin-duo, LI Zhe-ming. A new oleanane type triterpenoid from Viburnum taitoense Hayata[J]. Acta Pharmaceutica Sinica, 2019, 54(7): 1260-1264.

台东荚蒾中一个新的齐墩果烷型三萜
吴云秋1, 黄云峰2, 罗迪1, 邱莉1, 谢集照1,3, 徐焕基1, 武鑫铎1, 李哲明1
1. 广西医科大学药学院, 广西 南宁 530021;
2. 广西中医药研究院, 广西 南宁 530022;
3. 广西大学化学化工学院, 广西 南宁 530004
摘要:
采用硅胶柱色谱、Sephadex LH-20柱色谱等色谱方法,对台东荚蒾乙酸乙酯部位进行分离,从中分离纯化得到7个三萜类化合物,根据其波谱数据和理化性质鉴定为3β,6β-二羟基齐墩果-11,13(18)-二烯-28-酸(1)、3β-羟基齐墩果-11,13(18)-二烯-28-酸(2)、12-烯-齐墩果-28-酸-3β-棕榈酸酯(3)、3β-乙酰古柯二醇(4)、科索酸(5)、熊果醇(6)和熊果酸(7)。其中,化合物1为新化合物,其绝对构型通过单晶衍射得以证实。化合物2~5为首次从荚蒾属中分离得到。采用LPS诱导的RAW264.7释放NO的细胞模型,评价了化合物1~7的体外抗炎活性,结果显示,与阳性对照槲皮素(IC50值为25.46 ±0.62 μmol·L-1)相比,化合物5显示出较强的NO抑制活性,其IC50值为25.52 ±0.56 μmol·L-1
关键词:    荚蒾属      台东荚蒾      五环三萜      NO      RAW264.7     
A new oleanane type triterpenoid from Viburnum taitoense Hayata
WU Yun-qiu1, HUANG Yun-feng2, LUO Di1, QIU Li1, XIE Ji-zhao1,3, XU Huan-ji1, WU Xin-duo1, LI Zhe-ming1
1. School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China;
2. Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning 530022, China;
3. School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China
Abstract:
The chemical constituents of Viburnum taitoense Hayata were investigated using column chromatography silica gel and Sephadex LH-20, etc. Seven pentacyclic triterpenoids were isolated and their structures were elucidated by spectral data and physicochemical properties as 3β,6β-dihydroxy olean-11,13(18)-dien-28-acid (1), 3β-hydroxy olean-11,13(18)-diene-28-acid (2), 12-ene-olean-28-acid-3β-palmitate (3), 3β-acetylcocodiol (4), corosolic acid (5), uvaol (6) and ursolic acid (7). Among them, compound 1 is a new oleanane type triterpenoid and its absolute configuration was confirmed by single-crystal X-ray diffraction data. Compounds 2-5 were isolated from this genus for the first time. All the compounds were evaluated for their anti-inflammatory activities in vitro, and compound 5 showed significant inhibitory activity against nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells with an IC50 of 25.52 ±0.56 μmol·L-1 when compared to the positive control, quercetin (IC50 of 25.46 ±0.62 μmol·L-1).
Key words:    Viburnum    V. taitoense Hayata    pentacyclic triterpenoid    NO    RAW264.7   
收稿日期: 2019-04-23
DOI: 10.16438/j.0513-4870.2019-0316
基金项目: 南宁市科技局重点研发项目(20163162);广西科学研究与技术开发计划(桂科能1598025-3).
通讯作者: 邱莉,Tel:13878897164,E-mail:qlydmyyq@163.com
Email: qlydmyyq@163.com
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