药学学报, 2019, 54(7): 1271-1276
引用本文:
卢佳杰, 闫静, 许庆轩. 使用木尖喷雾和直接电离质谱对比分析制附子中乌头碱类生物碱[J]. 药学学报, 2019, 54(7): 1271-1276.
LU Jia-jie, YAN Jing, XU Qing-xuan. Comparative analysis of aconitine alkaloids in aconite tube using direct ionization and wooden tip spray mass spectrometry[J]. Acta Pharmaceutica Sinica, 2019, 54(7): 1271-1276.

使用木尖喷雾和直接电离质谱对比分析制附子中乌头碱类生物碱
卢佳杰1, 闫静1, 许庆轩2
1. 黑龙江中医药大学, 黑龙江 哈尔滨 150040;
2. 黑龙江大学, 黑龙江 哈尔滨 150081
摘要:
采用直接电离技术和木尖喷雾电离技术检测中药材制附子,建立快速、简便分析其中乌头碱类生物碱的方法。将制附子用水浸湿的滤纸包裹过夜,剪成三角形,滴加不同溶剂,分别用于直接电离质谱和木尖喷雾质谱系统检测。结果表明,这两种质谱分析方法不经过复杂的前处理过程,均能快速检测制附子中的生物碱,效果好于传统的毛细管电喷雾质谱分析方法。两种分析方法对比,除了以二氯甲烷为溶剂时,直接电离质谱分析的效果都好于木尖喷雾质谱。在不同极性的溶剂条件下,能选择性地检测出不同种类的生物碱。本实验为制附子成分分析提供了一种快速的质谱方法,从而为其种植、储存、炮制、配伍、品质的研究提供方法。
关键词:    电喷雾质谱      木尖喷雾      直接电离      制附子      生物碱     
Comparative analysis of aconitine alkaloids in aconite tube using direct ionization and wooden tip spray mass spectrometry
LU Jia-jie1, YAN Jing1, XU Qing-xuan2
1. Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China;
2. Heilongjiang University, Harbin 150081, China
Abstract:
In this manuscript, a rapid and simple analysis method for aconitine alkaloids was established. The method was based on the use of direct ionization and wooden tip spray ionization technology to detect the aconite. The aconite tuber slices were wrapped with wet filter paper overnight, cut into triangles, and extracted with a few solvents for direct ionization and wooden tip spray mass spectrometry. The results showed that alkaloids in aconite tuber can be rapid detected by two mass spectrometric methods without tedious sample pretreatment. Both methods are superior to that of traditional capillary electrospray ionization mass spectrometry (ESI-MS). The direct ionization MS is better than the wooden tip spray MS for analysis of aconite except under the condition of methylene chloride as extract or spray solvent. Different types of alkaloids in aconite tuber can be selectively detected when different solvents are used. The experiments provide a rapid and no pretreatment MS spectrometric method for analysis of alkaloids in aconite. These sample methods are important for research on aspects of plant varieties, storage, prescription compatibility, and quality control of aconite.
Key words:    ESI-MS    wooden tip spray    direct ionization    aconite tube    aconitine alkaloids   
收稿日期: 2019-02-24
DOI: 10.16438/j.0513-4870.2019-0131
基金项目: 黑龙江省自然科学基金资助项目(B2018011).
通讯作者: 闫静,Tel:13214615962,E-mail:yanjing_002@163.com;许庆轩,Tel:13895713386,E-mail:xuqx@hlju.edu.cn
Email: yanjing_002@163.com;xuqx@hlju.edu.cn
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