药学学报, 2019, 54(7): 1277-1287
引用本文:
曹宇欣, 李科, 秦雪梅, 焦思明, 杜昱光, 李树颖, 李先荣. 基于糖特征图谱及免疫细胞活性研究的不同产地黄芪的质量评价[J]. 药学学报, 2019, 54(7): 1277-1287.
CAO Yu-xin, LI Ke, QIN Xue-mei, JIAO Si-ming, DU Yu-guang, LI Shu-ying, LI Xian-rong. Quality evaluation of different areas of Astragali Radix based on carbohydrate specific chromatograms and immune cell activities[J]. Acta Pharmaceutica Sinica, 2019, 54(7): 1277-1287.

基于糖特征图谱及免疫细胞活性研究的不同产地黄芪的质量评价
曹宇欣1, 李科1,3, 秦雪梅1, 焦思明3, 杜昱光3, 李树颖1,2, 李先荣4
1. 山西大学中医药现代研究中心, 山西 太原 030006;
2. 山西大学化学化工学院, 山西 太原 030006;
3. 中国科学院过程工程研究所, 北京 100190;
4. 山西健硕食品药品研究院有限公司, 山西 太原 030000
摘要:
通过建立黄芪多糖和单糖糖谱,结合技术成熟的细胞免疫活性实验,建立以多糖为质控指标的黄芪品质评价方法。本研究采用高效液相色谱仪(HPLC)建立黄芪总多糖和单糖特征图谱,利用SIMCA软件和SPSS软件对数据分别进行多元统计分析和聚类分析以区分不同产地及不同种植方式的蒙古黄芪,并通过小鼠腹腔巨噬细胞吞噬中性红实验进行活性评价。结果表明山西浑源仿野生黄芪多糖含量和增强巨噬细胞吞噬活性的能力较高于移栽芪。山西浑源、山西五寨和甘肃陇西的黄芪多糖具有相似的分子量分布,但每个部分的峰面积占总峰面积的百分比具有明显差异,山西黄芪多糖中分子量为10 kDa左右的部分高于甘肃黄芪,PCA显示可以将山西黄芪和甘肃黄芪区分开。三者都是由鼠李糖、葡萄糖、半乳糖、阿拉伯糖和半乳糖醛酸5种单糖组成的。但3个产地的黄芪多糖(APS)具有不同的单糖物质的量比。PCA显示可以将3种不同的蒙古黄芪区分开。本研究采用了将糖谱技术与APS对细胞免疫功能的影响相结合的方法为不同产地及不同种植方式黄芪的品质评价及质量控制提供了依据。
关键词:    黄芪多糖      特征图谱      细胞免疫活性      仿野生      移栽      品质评价     
Quality evaluation of different areas of Astragali Radix based on carbohydrate specific chromatograms and immune cell activities
CAO Yu-xin1, LI Ke1,3, QIN Xue-mei1, JIAO Si-ming3, DU Yu-guang3, LI Shu-ying1,2, LI Xian-rong4
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
3. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
4. Shanxi Jianshuo Food and Drug Research Institute Co. Ltd., Taiyuan 030000, China
Abstract:
To establish a quality evaluation method for Astragali Radix using polysaccharide as quality control index, we established the Astragalus polysaccharide and monosaccharide sugar spectra, and combined with immunological activity test. High performance liquid chromatography (HPLC) was used to establish the specific chromatograms of Astragalus polysaccharides and monosaccharides. The data were analyzed by multivariate statistical analysis and cluster analysis using SIMCA software and SPSS software to distinguish Astragalus membranaceus var. mongholicus from different habitats or planting methods. The activity was evaluated by testing mouse peritoneal macrophage phagocytosis using neutral red. The results showed that the content of polysaccharides and the ability of enhancing phagocytic activity of macrophages from imitation wild Astragali Radix in Shanxi Hunyuan was higher than cultured Astragali Radix. The polysaccharides of Astragali Radix from Shanxi Hunyuan, Shanxi Wuzhai and Gansu Longxi have similar molecular weight distribution, but the peak area of each part has a significant difference in the percentage of the total peak area. The part of the polysaccharide of Shanxi Astragalus membranaceus with a molecular weight of about 10 kDa is higher than that of Gansu. Principal component analysis (PCA) shows that Astragali Radix from Shanxi and Gansu can be separated. All three are composed of five monosaccharides such as rhamnose, glucose, galactose, arabinose and galacturonic acid. However, the Astragalus polysaccharides (APS) in the three regions have different ratios of monosaccharide substances. The PCA display can distinguish three different Astragalus membranaceus var. mongholicus. This study used a combination of fingerprint of carbohydrates and the effects of APS on cellular immune function to provide a basis for quality evaluation and quality control of different habitats or planting methods.
Key words:    Astragalus polysaccharide    specific chromatogram    cellular immune activity    imitation wild    cultured    quality evaluation   
收稿日期: 2019-02-26
DOI: 10.16438/j.0513-4870.2019-0140
基金项目: 山西省优秀人才科技创新项目(201605D211030,201705D211020);山西省重点研发计划重点项目(201603D311101);国家中药标准化项目(ZYBZH-Y-JIN-34);山西省科技攻关项目(20150313004-5);国家自然科学基金资助项目(81872962).
通讯作者: 秦雪梅,Tel/Fax:86-351-7018379,E-mail:qinxm@sxu.edu.cn;李科,Tel/Fax:86-351-7019297,E-mail:like@sxu.edu.cn
Email: qinxm@sxu.edu.cn;like@sxu.edu.cn
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