药学学报, 2019, 54(8): 1457-1460
引用本文:
李国玉, 吕鑫宇, 菅计苹, 朱丹丹, 刘莹, 吕春艳, 郭玉岩, 吕邵娃. 万寿菊根中一个新的双取代丁二酸酯[J]. 药学学报, 2019, 54(8): 1457-1460.
LI Guo-yu, L� Xin-yu, JIAN Ji-ping, ZHU Dan-dan, LIU Ying, L� Chun-yan, GUO Yu-yan, L� Shao-wa. A new disubstituted succinate from the roots of Tagetes erecta[J]. Acta Pharmaceutica Sinica, 2019, 54(8): 1457-1460.

万寿菊根中一个新的双取代丁二酸酯
李国玉1, 吕鑫宇1, 菅计苹2, 朱丹丹1, 刘莹1, 吕春艳1, 郭玉岩2, 吕邵娃2
1. 哈尔滨商业大学药学院, 黑龙江省预防与治疗老年性疾病药物研究重点实验室, 黑龙江 哈尔滨 150076;
2. 黑龙江中医药大学中医药教育部重点实验室, 黑龙江 哈尔滨 150040
摘要:
采用硅胶柱色谱、Sephadex LH-20色谱等技术从万寿菊根的95%乙醇提取物中分离得到9个化合物,利用波谱法对其进行结构鉴定,分别为:5-羟甲基糠醛基-甲基-丁二酸酯(1)、5,7,3'-三羟基-3,6,4'-三甲氧基黄酮(2)、丁香酸(3)、5,7,4'-三羟基-3,6-二甲氧基黄酮(4)、万寿菊素-4'-甲氧基-7-O-β-D-葡萄糖苷(5)、万寿菊苷(6)、5,3'-二羟基-3,6,4'-三甲氧基-黄酮-7-O-β-D-葡萄糖苷(7)、2,2'-二联噻吩-5-醇(8)和3-羟基-4-甲氧基苯甲酸(9)。其中化合物1为新化合物,化合物8为首次从天然来源分离得到,化合物2459为首次在该属植物中分离得到。通过测定比较不同化合物对大豆胞囊线虫的生物活性及毒力,测得化合物1~8对大豆胞囊线虫均具有毒杀作用,化合物37的ED50均为0.008μg·mL-1,表现出更强的毒杀作用。
关键词:    万寿菊      丁二酸酯      提取分离      结构鉴定      杀虫活性     
A new disubstituted succinate from the roots of Tagetes erecta
LI Guo-yu1, L� Xin-yu1, JIAN Ji-ping2, ZHU Dan-dan1, LIU Ying1, L� Chun-yan1, GUO Yu-yan2, L� Shao-wa2
1. Heilongjiang Province Key Laboratory of Preventive and Therapeutic Drug Research of Senile Diseases, Pharmaceutical College, Harbin University of Commerce, Harbin 150076, China;
2. The Ministry of Education(MOE) Key Laboratory of Chinese Medicines, Heilongjiang University of Chinese Medicines, Harbin 150040, China
Abstract:
Nine compounds were isolated from 95% ethanol extract of the roots of Tagates erecta by silica gel column chromatography and Sephadex LH-20 chromatography. Their structures were identified by spectroscopic data as 5-hydroxymethylfurfuryl methyl succinate (1), 5,7,3'-trihydroxyl-3,6,4'-trimethoxylflavone (2), syringic acid (3), 5,7,4'-trihydroxyl-3,6-trimethoxylflavone (4), patuletin-4'-methoxyl-7-O-β-D-glucopyranoside (5), patulitrin (6), 5,3'-dihydroxyl-3,6,4'-trimethoxylflavone-7-O-β-D-glucopyranoside (7), (2,2'-biththiophen)-5-ol (8), and 3-hydroxyl-4-methoxyl benzoic acid (9). Among them, compound 1 is a new disubstituted succinate and compound 8 was isolated from a natural resource for the first time. Compounds 2, 4, 5 and 9 were isolated from this genus for the first time. By measuring the biological activity and virulence of different compounds against soybean cyst nematode, it has found that compounds 1-8 exhibited a toxic effect on soybean cyst nematode, and ED50 values indicate that compounds 3 and 7 are the most potent, with ED50 values of 0.008 μg·mL-1.
Key words:    Tagetes erecta    succinate    extraction and isolation    structure identification    insecticidal activity   
收稿日期: 2019-03-06
DOI: 10.16438/j.0513-4870.2019-0153
基金项目: 国家自然科学基金项目(81703724);哈尔滨商业大学博士启动基金(2019DS122);哈尔滨商业大学研究生科研创新项目(YJSCX2018-536HSD).
通讯作者: 吕邵娃,Tel:86-451-87266916,E-mail:lswa5599@sina.com
Email: lswa5599@sina.com
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